树突状细胞在变应性鼻炎实验小鼠发病机制中作用的研究

目的:探讨树突状细胞(DC)在变应性鼻炎实验小鼠发病中的可能作用及机制。方法:采用CpGoligodeoxynucleotide(CpGODN1826)培养诱导变应性鼻炎实验小鼠静脉血DC,进而检测DC协同刺激分子CD80、CD86的表达,以及其分泌的IL10、IL12等水平的变化。结果:实验小鼠静脉血DC其分泌IL10水平(3.6±0.5)μg/L明显低于对照组(6.8±0.8)μg/L,差异有统计学意义(t=20.608,P<0.01);分泌IL12水平(29.5±6.4)ng/L也明显低于对照组(46.2±9.8)ng/L,差异有统计学意义(t=5.979,P<0.01);同时,实验小鼠静脉血人类白细胞抗原DR(20.2±1.3)和协同刺激分子CD86的表达水平(36.8±13.5)明显高于对照组(11.6±1.5)和(26.7±8.5),差异有统计学意义(t=23.301和3.314,均P<0.01)。结论:DC可能通过诱导Th1/Th2细胞分化在变应性鼻炎实验小鼠发作时起重要作用,其可能的机制系通过DC分泌的细胞因子而起作用。

Role of dendritic cells in the pathogenesis of allergic rhinitis in mouse

OBJECTIVE: To investigate the role of dendritic cells in the pathogenesis of acute attack of allergic rhinitis in mouse. METHOD: The adherent precursors of DC were isolated from peripheral blood of allergic rhinitis mouse in acute attack stage and healthy controls. The adherent cells were induced with CpG ODN-1826 to DC in vitro. The expression of the surface molecules CD80, CD86, HLA-DR etc. on the DC was examined by fluorescent activated cell sorter (FACS), so the ability of DCs secrete IL-10, IL-12. RESULT: The results showed that the allergic rhinitis mouse in acute attack stage had remarkably decreased the ability to secret IL-10 compared with normal control subjects (P <0.01), while the ability to secret IL-12 remarkably decreased compared with normal control subjects (P < 0.01); meanwhile, the HLA-DR and co-stimulating factor CD86 expressed by DCs remarkably increased in the allergic rhinitis mouse in acute attack stage compared with the normal control subjects (P < 0.01). CONCLUSION: DC possibly plays a vital role in the immunological mechanism of allergic rhinitis by means of inducing the differentiation of Th1/Th2, that is DC may be the key factor in initiating the airway allergic reaction and the possible mechanism may involve interleukins (especially IL-10 and IL-12, etc.) secreted by DCs.