多发性创伤后单核细胞功能变化的实验研究

背景血浆纤维结合蛋白(Fn)是单核吞噬细胞吞噬功能的调节剂.多发伤后,血浆Fn发生明显有规律改变.有报道体外循环犬中外周血单核细胞活性增加.而多发伤后细胞Fn变化不清楚.目的检测多发伤犬外周血单核细胞其表面纤维结合蛋白(Fn)和吞噬功能.设计随机对照实验研究.地点和对象对随机造模的多发伤犬16只进行实验,年龄1～4年,平均为2.5年.干预用梯度离心法收集16例犬外周血单核细胞.主要观察指标多发伤犬单核细胞吞噬功能,表面Fn及血浆Fn水平变化.结果多发伤犬单核细胞吞噬功能在伤后第1,3,7天分别为(0.263±0.048),(0.388±0.087),(0.472±0.137)A,单核细胞吞噬功能7d显著性增强(t=1.07,P＜0.05);血浆Fn水平在伤后第3天(0.144±0.002)g/L和7天(0.152±0.003)g/L较第1天(0.125±0.009)g/L显著升高.单核细胞吞噬功能与血浆Fn水平显著相关(r=0.099,P=0.05).单核细胞表面Fn变化无显著性意义.结论多发伤后单核细胞表面Fn呈动态变化,与其吞噬功能和血浆变化相一致,其对评价创伤后单核细胞功能变化具有重要价值.

Experimental study onthe function of monocytes after multiple injuries

BACKGROUND: Plasma fibronectin (Fn) is the regulator of phagocytic function of mononuclear phagocytes. After multiple injuries, obvious and regular changes will happen to plasma Fn. It has been reported that the monocytic activity in peripheral blood increased in in vitro circulation of dogs, while the changes of Fn in the cells are still not clear.OBJECTIVE: To detect the Fn on the surface of monocytes and mononuclear phagocytic functions in the peripheral blood of dogs with multiple injuries.DESIGN: A randomized controlled experimental study.SETTING and PARTICIPANTS: Sixteen dogs with multiple injuries that were made randomly were used in the experiment. These dogs were 1 -4 years old, with an average age of 2.5 years.INTERVENTION: Monocytes in the peripheral blood of the dogs were collected by gradient centrifugation.MAIN OUTCOME MEASURES: Mononuclear phagocyte function of the dogs with multiple injuries; Fn on the surface of monocytes; plasma Fn.RESULTS: The phagocyte function of monocytes of the dogs with multiple injuries was (0. 263 ± 0. 048), (0. 388 ± 0. 087), (0. 472 ± 0. 137) respectively on days 1, 3, 7 after the injuries and it was strengthened obviously on day 7 after the injuries ( t = 1.07, P ＜ 0. 05); the plasma Fn level was (0. 144 ± 0. 002) g/L on day 3 after the injuries, and it was (0. 152 ±0. 003) g/L on day 7 after the injuries. These were obviously higher than that of the first day(0. 125 ±0.009) g/L. The mononuclear phagocyte function was significantly correlated with the plasma Fn level( r =0. 099,P = 0. 05 ) . The changes of Fn on the surface of monocytes showed no significant difference.CONCLUSION: The Fn on the monocyte surface showed dynamic changes after the injuries and this was consistent with the changes of the phagocyte function and the plasma Fn level. The results are of important value in evaluating the changes of monocytic function after the injuries.