霉酚酸酯及地塞米松对血管内皮细胞功能的影响

目的：研究霉酚酸酯，地寒米松对血管内皮细胞功能的影响，进一步探讨这两种免疫抑制剂抗炎作用的机制。方法：以脐静脉来源的的内皮细胞为研究对象，通过建立体外内皮细胞血管发生（ａｎｇｉｏｇｅｎｅｓｉｓ）模型，观察并比较了两种免疫抑制剂对内皮细胞血管发生，内皮细胞迁移能力（Ｓｃｒａｔｃｈ ｗｏｕｎｄ ｃｌｏｓｕｒｅ ａｓｓａｙ）和内皮细胞增生（＾３Ｈ－ＴｄＲ掺入）的影响。结果：霉酚酸酯对血管内皮细胞体外血管

INFLUENCE OF MYCOPHENOLATE MOFETIL AND DEXAMETHASONE ON THE FUNCTION OF ENDOTHELIAL CELLS

To explore the effect of mycophenolate mofetil(MMF) and dexamethasone(DEX) on endothelial cells. METHODOLOGY A cell line of human umbilical vein endothelial cells (HUVEC) from ATCC was used in the study. In vitro angiogenesis assay model was established to verify the effect of MMF and DEX on the angiogenic activity in HUVEC. The migration ability of HUVEC was measured by the scratch wound closure assay and the cell proliferation was determined by 3H TdR incorporation assay. RESULTS MMF and DEX were found to inhibit angiogenesis of HUVEC in vitro, and this effect was more potent in MMF. The migration and proliferation ability of HUVEC was inhibited significantly by MMF, but not DEX. CONCLUSION As an immunosuppressant, MMF is not only effect on the lymphocytes, but also inhibit the angiogenesis, cell migration and proliferation of endothelial cells. These effects of MMF are quite different from dexamethason.