端粒酶活性与膀胱肿瘤T24细胞的诱导分化研究

目的：探讨人膀胱肿瘤T24细胞诱导分化治疗与端粒酶活性改变的关系。方法：用全反式维甲酸（ATRA）诱导分化人膀胱肿瘤T24细胞，用聚合酶链反应-酶联免疫吸附试验（PCR-ELISA）法检测T24细胞诱导分化后端粒酶活性变化，用流式细胞术检测细胞周期动力学改变。结果：T24细胞经ATRA诱导分化后，细胞周期动力学发生改变：S期细胞所占比例逐渐降低，G0/G1期细胞所占比例逐渐增加；端粒酶活性被明显抑制，与对照组比较，ATRA处理后1、3、5、7d端粒酶活性抑制率分别为10.9％、34.7％、81.2％、92.5％；端粒酶活性的高低与细胞所处的分化状态有关。结论：人膀胱肿瘤T24细胞诱导分化后，其细胞周期动力学发生改变，端粒酶活性被抑制，这可能为膀胱肿瘤的诱导分化治疗提供理论依据。

Telomerase activity and differentiation of T24 cell lines

Objective To investigate the relationship between induction of differentiation of T24 cell lines and alteration of telomerase activity.Methods T24 cell lines were induced by all trans retinoic acid (ATRA);Telomerase activity was measured by Polymerase Chain Reaction Enzyme Linked Immunosorbent Assay(PCR ELISA) after induction of T24 cell lines;Cell cycle was detected by flow cytometry (FCM).Results The cell cycle dynamics of T24 cell lines was changed signifiacntly after induction of differentiation with ATRA:the ratio of stage S cells decreased gradually,while the ratio of stage G 0/G 1 cells increased gradually;telomerase activity was significantly inhibited with the inhibitory ratios of telomerase activity being 10.9%,34.7%,81.2%,92.5% respectively after treated with ATRA for 1,3,5,7 day,which were compared with control group.Telomerase activity wasn't inhibited by differentiation inducing agents directly,which indicated that the inhibition of telomerase activity responded to the induction of differentiation and the loss of telomerase activity was colsely associated with the state of the celluar differentiation.Conclusion The cell cycle dynamics was changed and the telomerase activity was inhibited after T24 cell lines induced by ATRA,which could provide the theoretical basis of bladder carcinoma differentiation inducing therapy.