改良Cuff技术建立小鼠颈部异位心脏移植模型

 目的 改进Cuff技术制作小鼠颈部异位心脏移植模型，以简化手术操作，提高手术成功率。方法 使用自制套管将供心肺动脉套接于受体右颈外静脉，将供心升主动脉套接于受体右颈总动脉。结果 正式实验40例，手术成功率92.5%。手术时间约60min，供心冷缺血时间小于30min。结论 改良Cuff技术建立小鼠颈部异位心脏移植模型，无需显微外科操作，是一种经济实用、稳定可靠、易于复制的动物模型。

A model of cervical heterotopic heart transplantation in mice by modified Cuff technique

Objective To simplify surgical technique and increase postoperative survival rate by using improved Cuff technique in making the model of cervical heterotopic heart transplantation in mice. Methods By using self-made cuffs,the donor pulmonary artery was anastomosed to the recipient right external jugular vein,the donor ascending aorta was anastomosed to the recipient right common carotid artery. Results Forty formal transpamtations were performed with a successful rate of 92.5%.The operative time was about 60 min,the cold ischemic time for donor heart was shorter than 30 min. Conclusions The model of cervical heterotopic heart transplantation in mice by modified Cuff technique is an economical,practicable,reliable and high reproducible animal model,and can be operated by surgeons without professional microsurgery technique.