植雌素防治骨质疏松症的实验研究

目的观察植雌素(phytoestrogen)对去卵巢大鼠骨质疏松症的防治作用。方法45只3月龄Wistar雌性大鼠，随机分为假手术组、模型组、植雌素低剂量组(简称低剂量组)、植雌素高剂量组(简称高剂量组)和雌二醇组，每组9只。假手术组仅行假手术，其余4组均行卵巢切除术，术后第31天开始给药，假手术组和模型组每天灌服生理盐水2m1，植雌素高、低剂量组每天分别灌服植雌素10mg／kg、5mg／kg，雌二醇组每周肌肉注射1次雌二醇0．1mg／kg。连续用药90天后，检测血清生化指标，剥离右股骨测定骨密度，取左股骨做骨小梁病理形态学检测。完整摘取子宫称重，计算子宫指数。结果不同剂量植雌素及雌二醇均可增加去卵巢大鼠的血清雌二醇浓度、血磷含量，并能提高子宫重量及子宫系数；降低血清骨钙素、血浆抗酒石酸酸性磷酸酶、血清碱性磷酸酶和白介素-6含量，与模型组比较差异有显著性(P<0．05或P<0．01)。病理形态学观察发现：模型组骨皮质变薄，骨小梁变细，完整性差，扭曲、断裂、面积减少，而不同剂量植雌素组及雌二醇组上述变化明显改善。结论植雌素对去卵巢大鼠骨质疏松症有防治作用。

Experimental Study on Prevention and Treatment of Osteoporosis by Phytoestrogen

To observe the effect of phytoestrogen (PE) in preventing and treating osteoporosis in ovariec-tomized rats. Methods Forty-five Wistar female rats, 3 months old, were randomly divided into the sham-operated group, the model group, low dose and high dose PE groups, and the positive control group, 9 in each group. All rats, except that those in the sham operated group were simutaneously operated, were ovariec-tomized. The medication started from the 31st days after operation by daily given saline per os to the sham-operated group and the model group, PE per os to the PE groups, and estradiol by intramuscular injection to the positive control group. The animals were sacrificed 90 days later, their sera were collected for determination of biochemical parameters, their right femoral bone was taken to detect the bone mineral density (BMD), left femoral bone to examine pathology of bone trabecula, and whole uterus was weighed to calculate the uterine index. Results PE could increase the BMD level, serum estradiol and inorganic phosphorus content, and uterine index, lower serum BGP, tartrate-resistant acid phosphatase (TRAP), alkaline phosphatuse ( AKP) and interleukin-6 levels. Pathological examination showed that in the model group, the cortex of bone thinned, bone trabecula thinned also, with poor integrity, distorted, broken and decreased in size, while in the PE groups, the above-mentioned changes were significantly alleviated. Conclusion PE is effective in preventing and treating osteoporosis in ovariectomized rats.