PML-RARα多肽诱导脐血TCR Vβ T细胞增殖研究

 目的 了解PMLRARd多肽诱导脐血T细胞的T细胞受体（TCR）V8谱系表达和克隆性情况。方法 以不同浓度（16．7μg／mL，33．3μg／mL或50μg／mL）的PML-RARα多肽联合IL-2、抗CD3单抗以及抗CD28单抗诱导脐血T细胞增殖，利用RT-PCR-基因扫描技术分析诱导后第3、6、9、12天后的脐血TCRV8亚家族的利用和克隆性增殖的特点。结果 限制性表达和克隆性增殖TCR Vβ亚家族T细胞可见于PML-RARα多肽诱导后脐血T细胞。结论 PML-RARα多肽可在体外诱导脐血T细胞呈克隆性增殖，此优势增殖的克隆性T细胞可能具有特异性细胞毒作用。

Clonal Expansion of TCR Vβ Subfamily T Cells Induced by PML-RARα Peptide

Objective 　To investigate the expression and clonality of T cell receptor ( TCR) Vβ repertoire in cord blood T cells induced by PML-RARα peptide in vitro. Methods 　Cord blood mononuclear cells were amplified by IL-2 , anti-CD3 and anti-CD28 antibody with different concent ration (16. 7μg / mL ,33. 3μg / mL or 50μg / mL respectively) of synthetic PML-RARαpeptide. The induced T cells were collected at different time point s af ter culture (3 , 6 , 9 or 12 days) . The expression and clonality of TCR Vβ subfamilies within induced T cells were analyzed by using RT-PCR and genescan technique. Results 　Restricted expression and cloanl expansion of TCR Vβ subfamily cord blood T cells could be identified afterinduction by PML-RARαpeptide. Conclusion 　The PML-RARα peptide could induce the clonal expansion T cells from cord blood in vitro , which may have specific cytotoxicity for PML-RARα+ cells.