反相高效液相色谱法测定人血浆中噻奈普汀钠的浓度

目的 :用高效液相色谱法测定人血浆中噻奈普汀钠的浓度。方法 :以DiamonsilTM C18 反相柱 (4 6mm×250mm ,5μm )为色谱柱 ,流动相为甲醇 -0 3mol/L乙酸铵 (80∶20) ,流速为0 8ml/min ,检测波长为220nm ,以乙酸乙酯为提取剂。结果 :噻奈普汀钠高、中、低3种浓度的平均回收率分别为97 63 %、97 60%、93 67 % ,日间差精密度均小于5 % (n=5) ;分析方法的定量测定下限为0 2μg/ml。线性范围为1 0～83 33μg/ml,回归方程为Y=6 05×10-2X +8 18×10-1 ,r=0 9994(n=12)。结论 :本方法操作简单、快速 ,检测结果准确 ,灵敏度高 ,重现性好 ,可用于临床血药浓度监测和药代动力学研究。

Determination of the Contents of Tianepine Sodium in Blood Plasma by RP-HPLC

OBJECTIVE:To develop HPLC method for quantitative determination of ti anepine sodium in blood plasma METHODS:Tianepine was extracted with ethyl acet ate The residues were analyzed with a reverse phase HPLC system(DiamonsilTM C18 column,4 6mm×250mm,5μm);Mobile phase,MeOH-C2H3OONH4(80∶20);UV detecti on 220nm RESUL_TS:The average recoveries for tianepine sodium in high,medium and low concentr ations were 97 63%,97 60% and 93 67%,respectively The within-day and b etween-day relative standard deviations were less than 5%(n=5) The calibratio n curves had good linearity within a concentration range of 1 0～83 3μg/ml T he regression equation was Y=6 05×10-2X+8 18×10-1,r=0 9 994(n=12) The limit of quantitation for tianepine sodium was 0 2μg/ml CONCLUSION:The metho d provides a se_nsitive,accurate,precise and reliable analytical procedure for clinical monito ring of tianepine sodium blood in plasma and its pharmacokinetic studies