| 大鼠变应性鼻炎模型中T-bet和GATA-3的表达 |
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| 作者姓名： | Li Y Chen D Li T Wen W Shi J He X |
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| 作者单位： | 1. 昆明医学院附属第一医院耳鼻咽喉头颈外科,昆明,650031
2. 中山大学附属第一医院耳鼻咽喉科 |
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| 基金项目： | 广东省自然科学基金(No:8151008901000105) |
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| 摘 要： | 目的:探讨在大鼠变应性鼻炎(AR)模型单核细胞中T-bet/GATA-3基因的动态表达。方法:20只SD大鼠,雌雄各半,随机分为实验组和对照组,每组各10只。实验组用卵蛋白致敏,以氢氧化铝溶胶和灭活的百日咳杆菌为佐剂,卵蛋白滴鼻建立大鼠AR模型;对照组以氢氧化铝溶胶和灭活的百日咳杆菌生理盐水皮下注射,生理盐水滴鼻。分别在致敏结束、首次激发10h、末次激发10h心脏采血,分离血单核细胞。用RT-PCR法测定单核细胞中T-bet/GATA-3mRNA的表达,对结果进行统计学分析。结果:致敏结束、首次激发10h、末次激发10h3个时间点实验组T-bet mRNA的相对表达量分别为0.404±0.187、1.676±0.708、0.503±0.514,GATA-3mRNA的相对表达量分别为0.434±0.147、0.600±0.480、1.029±0.690;对照组T-bet mRNA的相对表达量分别为0.487±0.212、0.486±0.148、0.495±0.103,GATA-3mRNA的相对表达量分别为0.596±0.249、0.474±0.101、0.550±0.119。实验组首次激发后10hT-bet m...
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| 关 键 词： | T-bet GATA-3 鼻炎 变应性 动物模型 |
Kinetic expression of T-bet and GATA-3 genes in rat allergic rhinitis models |
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| Li Y,Chen D,Li T,Wen W,Shi J,He X.Kinetic expression of T-bet and GATA-3 genes in rat allergic rhinitis models[J].Journal of Clinical Otorhinolaryngology,2011,25(17):791-795. |
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| Authors: | Li Yuxiao Chen Dong Li Tianying Wen Weiping Shi Jianbo He Xiaoguang |
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| Institution: | LI Yuxiao1 CHEN Dong2 LI Tianying2 WEN Weiping2 SHI Jianbo2 HE Xiaoguang1(1Department of Otolaryngology-Head and Neck Surgery,the First Affiliated Hospital of Kunming Medical College,Kunming,650031,China,2Department of Otolaryngology,the First Affiliated Hospital of Sun Yet-san University) |
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| Abstract: | Objective:To investigate kinetic expression of genes T-bet and GATA-3 in blood mononuclear cells(BMCs)in allergic rhinitis rats model at different stages of development of allergic rhinitis.Method:Twenty SD rats(including 10 male and 10 female)were divided into 2 groups,experimental group and control group,randomly,10 rats for each group.Ten rats in experimental group were sensitized and intranasally challenged by OVA,aluminium hydroxide hydrate gel and Bordelella pertussis inactive microorganism suspension... |
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| Keywords: | T-bet GATA-3 rhinitis allergic animal model |
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