| ARHI基因对胰腺癌细胞凋亡的影响 |
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| 引用本文： | 路新卿,李桂英,王德峰,蔡莉,王友明,李校天,刘志军. ARHI基因对胰腺癌细胞凋亡的影响[J]. 临床荟萃, 2011, 26(24): 2145-2148 |
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| 作者姓名： | 路新卿 李桂英 王德峰 蔡莉 王友明 李校天 刘志军 |
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| 作者单位： | 河北工程大学附属医院 消化内科,河北邯郸,056002 |
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| 基金项目： | 河北省高等学校科学技术研究青年基金项目(2010118); 河北省医学科学研究重点课题计划(20110156) |
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| 摘 要： | 目的研究ARHI基因对人胰腺癌细胞株PANC-1和MiaPaCa-2凋亡的影响,探讨其诱导凋亡的作用机制。方法分别应用反转录聚合酶链反应（RT—PCR）和免疫印迹法（Westernblot）检测稳定转染的胰腺癌细胞中ARHI和死亡相关蛋白激酶1（DAPK-1）的mRNA和蛋白表达,应用流式细胞仪检测稳定转染的胰腺癌细胞中发生细胞凋亡的百分率。结果转染ARHI和空载体质粒后,无转染组和空载体组均无ARHImRNA和蛋白的表达,ARHI组可见ARHImRNA和蛋白的表达;ARHI组PANC-1、MiaPaCa-2细胞凋亡的百分率分别为（32．9±6．7）％和（39．5±6．0）％,均高于空载体组的（13．8±2．9）％和（15．2±1．8）％（P〈0．01）;在稳定转染的PANC-1和MiaPaCa-2细胞中,ARHI组DAPK-1基因和蛋白表达均较空载体组和无转染组上调。结论ARHI可明显的诱导胰腺癌细胞发生凋亡,DAPK-1参与了ARHI引起凋亡的机制。ARHI表达下调可能在胰腺癌的发生发展中起了重要的作用。
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| 关 键 词： | 胰腺肿瘤 基因 细胞凋亡 细胞培养技术 逆转录聚合酶链反应 |
Mechanism of apoptosis induced by ARHI gene in pancreatic cancer cells |
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| LU Xin-qing,LI Gui-ying,WANG De-feng,CAI Li,WANG You-ming,LI Xiao-tian,LIU Zhi-jun. Mechanism of apoptosis induced by ARHI gene in pancreatic cancer cells[J]. Clinical Focus, 2011, 26(24): 2145-2148 |
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| Authors: | LU Xin-qing LI Gui-ying WANG De-feng CAI Li WANG You-ming LI Xiao-tian LIU Zhi-jun |
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| Affiliation: | LU Xin-qing,LI Gui-ying,WANG De-feng,CAI Li,WANG You-ming,LI Xiao-tian,LIU Zhi-jun Department of Gastroenterology,the Affiliated Hospital of Hebei University of Engineering,Handan 056002,China |
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| Abstract: | Objective To study the effects of apoptosis of human pancreatic cancer cell line PANC-1 and MiaPaCa-2 cells induced by ARHI gene and to investigate its mechanism.Methods The mRNA and protein expression of ARHI and death associated protein kinase 1(DAPK-1) were analyzed by reverse transcriptase polymerase chain reaction(RT-PCR) and Western blot methods respectively in stable transfected pancreatic cells.Flow cytometry was used to analyze the effect of ARHI on the apoptosis of stable transfected cell line.Res... |
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| Keywords: | pancreatic neoplasms genes apoptosis cell culture techniques reverse transcriptase polymerase chain reaction |
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