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鱼腥草抗病毒有效成分槲皮素的含量测定
引用本文:张柳红,李丹,李力.鱼腥草抗病毒有效成分槲皮素的含量测定[J].山西中医,2010,26(5):48-49,51.
作者姓名:张柳红  李丹  李力
作者单位:1. 湖北中医学院,武汉,430022
2. 湖北省武汉市中西医结合医院
基金项目:湖北省卫生厅资助项目 
摘    要:目的:运用高效液相色谱法测定临床治疗孕产妇巨细胞病毒感染的鱼腥草抗病毒有效成分槲皮素的含量。方法:采用Agilent ZORBAX ODS色谱柱,以甲醇-0.4%磷酸(52∶48)为流动相;368 nm波长处检测,流速:1.0 mL/min,柱温:30℃。结果:线性范围为0.24-1.20μg,相关系数r=0.9973,平均回收率为98.75%,RSD=0.66%。结论:该方法简便、准确,可以用于对鱼腥草中槲皮素含量进行检测。

关 键 词:鱼腥草  槲皮素  抗病毒  高效液相色谱法  含量测定

Quercetin Assaying of the Active Component in Houttuynia Cordata Thunb.
ZHANG Liu-hong,LI Dan,LI Li.Quercetin Assaying of the Active Component in Houttuynia Cordata Thunb.[J].Shanxi Journal of Traditional Chinese Medicine,2010,26(5):48-49,51.
Authors:ZHANG Liu-hong  LI Dan  LI Li
Institution:(Hubei TCM College,Wuhan,430022,China)
Abstract:Objective: To determine cont.of quercetin,the active component in houttuynia cordata(Thunb.)by HPLC,which can heal cytomegalovirus infection in pregnant and puerperant women.Method: HPLC was used in the quantitative analysis of quercetin in the sample.The separation was performed on Agilent ZORBAX ODS(4.6 mm×250 mm,5 μm) column at 30℃ with a mixture of methanol-0.4% phosphoric acid(52∶48) as mobile phase.The detection wavelength was set at 368 nm and the flow rate was 1.0 ml·min-1.Result: The linear range of quercetin was 0.24 μg-1.20 μg(r=0.997 3),the average recovery was 98.75%,RSD = 0.66%.Conclusion: The method is simple,accurate,and reliable in determining quercetin in houttuynia cordata Thunb..
Keywords:houttuynia cordata Thunb    quercetin  anti-virus  high performance liquid chromatography(HPLC)  assaying
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