| 高效液相色谱法测定肝泰胶囊中水飞蓟宾的含量 |
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| 引用本文： | 王雯佶,张纯,檀密艳,高申. 高效液相色谱法测定肝泰胶囊中水飞蓟宾的含量[J]. 中国药房, 2006, 17(22): 1736-1737 |
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| 作者姓名： | 王雯佶 张纯 檀密艳 高申 |
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| 作者单位： | 1. 第二军医大学长征医院药学部,上海市,200003
2. 第二军医大学药学院,上海市,200433 |
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| 摘 要： | 目的建立以高效液相色谱法测定肝泰胶囊中水飞蓟宾含量的方法。方法色谱柱为DiamonsilC18,流动相为磷酸二氢钾-甲醇(45∶55),流速为1·0ml/min,检测波长为288nm,内标为山奈酚,柱温为(25±2)℃,进样量为20μl。结果水飞蓟宾检测浓度在0·126～1·26mg/ml范围内线性关系良好(r=0·9998),平均加样回收率为102·9%(RSD=1·50%)。结论本方法简便、准确、重现性好,可用于本品的含量测定。
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| 关 键 词： | 高效液相色谱法 肝泰胶囊 水飞蓟宾 含量 |
| 文章编号： | 1001-0408(2006)22-1736-02 |
| 收稿时间： | 2006-01-23 |
| 修稿时间： | 2006-02-23 |
Assaying of Silybin in Gantai Capsule by HPLC |
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| WANG Wenji,ZHANG Chun,TAN Miyan,GAO Shen. Assaying of Silybin in Gantai Capsule by HPLC[J]. China Pharmacy, 2006, 17(22): 1736-1737 |
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| Authors: | WANG Wenji ZHANG Chun TAN Miyan GAO Shen |
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| Abstract: | OBJECTIVE:To establish an HPLC method for the assaying of silybin in Gantai capsule.METHODS:The assay was performed on Diamonsil C18 column.The mobile phase consisted of potassium dihydrogen phosphate solution-methanol(45:55)with a flow rate at 1.0ml/min,detection wavelength at 288nm,column temperature at(25±2)℃.The sample size was 20μl and the internal standard was kaempferol.RESULTS:There was a good linear relationship within the concentration range of 0.126～1.26 mg/ml(r=0.9 998)for silybin.The average recovery was 102.9%(RSD=1.50%).CONCLUSION:The method was simple,accurate and reproducible,and suitable for the assaying of silybin in Gantai capsule. |
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| Keywords: | HPLC Gantai capsule Silybin Content |
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