通窍止咳液体外抗甲型人流感病毒作用研究

目的:研究通窍止咳液体外抗甲型人流感病毒的作用。方法:以利巴韦林作阳性对照药物,采用存留细胞结晶紫染色法测定通窍止咳液不同加药方式对甲型人流感病毒H3N2的体外抑制作用及其时效关系。结果:通窍止咳液对甲型人流感病毒具有良好的综合抑制作用及抑制病毒吸附后的复制增殖作用;通窍止咳液对病毒吸附的预防作用弱,无直接杀伤病毒的作用;随药物作用时间延长,通窍止咳液在低浓度时的抗病毒有效率呈降低趋势,在高浓度时的抗病毒能力基本不变。结论:通窍止咳液具有较好的体外抗甲型人流感病毒的作用。

Antiviral Effects of Tongqiao Zhike Liquid against Human Influenza A Virus in Vitro

OBJECTIVE： To investigate the anti- viral effects of Tongqiao zhike liquid against influenza A virus in vitro. METHODS： The inhibitive effect of Tongqiao zhike liquid administered by different ways against human influenza A virus （H3N2） in vitro and its time - effect relationship were assayed by crystal violet staining assay with ribavirin as positive control. RESULTS： Tongqiao zhike liquid showed a satisfactory comprehensive inhibitory effect against human influenza A virus as well as its proliferation after viral adsorption. It was found that Tongqiao zhike liquid had a weak effect in preventing viral adsorption and it had no direct antiviral effect. The inhibitory effect of low concentration of Tongqiao zhike liquid against influenza A virus weakened time - dependently, while at high concentration its anti - viral ability remained stable. CONCLUSION： Tongqiao zhike liquid has a satisfactory effect against human influenza A virus in vitro.