多柔比星葡聚糖纳米粒的制备及其对卵巢癌细胞SKOV3的杀伤作用

目的:制备载多柔比星葡聚糖纳米粒(DOX/DEX-PLA),观察其药物缓释规律,探讨其对卵巢癌细胞的杀伤作用。方法:以双乳化剂蒸发法制备DOX/DEX-PLA载药纳米粒,透射电镜观察纳米粒形态,分光光度法计算载药率,体外药物释放实验考察纳米粒对DOX的缓释作用。MTT法观察对卵巢癌细胞的体外杀伤作用,动物实验观察其体内抑瘤效应。结果:DOX/DEX-g-PLA纳米微粒呈球形,粒径约83 nm,药物包封率约67.1%。体外释放实验提示,纳米制剂中约50%的药物持续缓慢释放,可持续达7 d;体内抑瘤实验显示,纳米缓释制剂间隔给药疗效优于未包载药物每日给药的疗效。结论:DOX/DEX-PLA纳米粒可有效抑制卵巢癌细胞的生长。

Preparation and Anti-Ovarian Cancer Effect of Doxorubicin Encapsulated in Amphiphilic Polysaccharide Nanoparticles

Objective: To investigate the preparation of DOX/DEX-PLA nanoparticles and their effect of killing ovarian cancer cells in vitro and in vivo.Methods: The DOX/DEX-PLA nanoparticles were prepared by the method of emulsification and evaporation of organic solvent.The morphology was observed by transmission electron microscopy,and the encapsulating efficiency of DOX was determined by ultraviolet spectrophotometry.The inhibitory effect against ovarian cancer cells in vitro was measured by MTT assay,and the anti-ovarian cancer effect in vivo was tested by animal experiment.Results: The DOX /DEX-PLA nanoparticles were of round or elliptical shape.The diameter of the nanoparticles was 83 nm,and the entrapment efficiency was 67.1%.The releasing test in vitro manifested that over 50 percent of DOX encapsulated in DOX /DEX-PLA nanoparticles were sustained released for about 7 d.As a result,the anti-ovarian cancer effect in vivo indicated that the efficacy of control-releasing agent by interval drug delivery has advantages over that of the unenveloped drug daily delivery.Conclusion: DOX /DEX-PLA nanoparticles can effectively inhibit the growth of ovarian cells.