实验性骨质疏松病理学观察及血清NO和NOS测定及其意义

目的观察去势雌性大鼠骨质疏松骨形态及骨计量学改变,测定血清NO和NOS水平,探讨NO和NOS在骨质疏松发病机制中的作用和意义。方法将45只3月龄雌性大鼠随机分成假手术组(Sham组);去势组(OVX组);尼尔雌醇/左炔诺孕酮治疗组(OVX+N/L组)。OVX+N/L组为去势后3天开始尼尔雌醇/左炔诺孕酮治疗。三组动物术后14周全部处死,通过图像分析仪对骨组织进行形态计量分析及血清NO和NOS的测定,并进行比较分析。结果OVX组较Sham组及OVX/NL组:相对骨小梁体积及骨小梁平均厚度显著减少(P<0.01);骨小梁吸收面积及成骨细胞覆盖骨小梁表面百分比明显增加(P<0.01);血清NO和NOS的水平显著降低(P<0.01)。OVX+N/L组较Sham组上述指标均无显著性差异(P>0.05)。结论去势雌性大鼠骨丢失是骨转换加快(高转换型)和重建负平衡骨吸收超过骨形成所致,血清中NO和NOS的变化可能发挥着重要作用;雌激素防止这种骨转换的负平衡可能通过NO和NOS途径来实现的。

The Pathological Observation of experimental osteoporosis and mensuration of NO and NOS in serum

Objective To observe the morphological and quantitative changes of osteoporosis in emasculated female rats, and mensturate the level of NO and NOS in serum so as to study the role and significance of NO and NOS in Osteoporosis.Methods 45 three moths old female rats were sorted into three groups randomly, nameed control group(Sham group) emasculated group(OVX group) and drug treatment group(OVX+N/L group), drug was used from the 3th day after be emasculated) All rats in three groups were killed at fourteenth week after operation. Bone's morphological and quantitative changes were analyzed the by image analyzer and the level of NO and NOS in serum were mensturated, then compared the difference among groups.Results OVX group compared with control group and OVX+N/L group: The volume of relative osteal girder and average thickness of osteal girder decreased remarkably (P<0.01); The absorptive acreage of osteal girder and percentage of osteoplast covering osteal girder was increased obviously(P<0.01); The level of NO and NOS in serum decreased remarkably(P<0.01). There was no statistical significance between OVX+N/L group and control group in above mentioned aspects (P>0.05).Conclusion The reason of bone-decrease in emasculated female rats is the negative balance between osteal conversion and rebuilding-bone absorption is quicker than bone rebuild. The change of NO and NOS in serum perhaps plays an important role in the course. Estrogen possibly regulates the level of NO and NOS in serum to prevent negative balance of bone absorption. 【