N-乙酰半胱氨酸对急性呼吸窘迫综合征肺纤维化的抑制作用

目的 从细胞水平观察N-乙酰半胱氨酸(NAC)对脂多糖(LPS)诱导氧自由基损伤致急性呼吸窘迫综合征(ARDS)肺纤维化形成的影响.方法 体外培养人胚肺成纤维细胞,分为空白对照组、LPS刺激组、NAC处理组、地塞米松(DEX)处理对照组4组,分别加入不含处理因素的培养液及含有LPS(1μg/ml)、NAC(1 mmol/L) +LPS(1μg/ml)、DEX(1μmol/L)+LPS(1 μg/ml)的培养液.培养24 h后测定细胞内胶原蛋白和还原型谷胱甘肽(GSH)的含量,以评价各因素对肺纤维化的影响.结果 与空白对照组比较,LPS刺激组肺成纤维细胞中胶原蛋白含量(μg/mg)显著增加(78.97±1.79比72.90±1.70,P＜0.05),GSH含量(μg／mg)显著减少(23.27土0.92比26.34±0.83,P＜0.05);NAC与DEX均可明显抑制LPS的作用(胶原蛋白含量:72.23±1.35、73.64±1.89比78.97±1.79;GSH含量:26.52±0.62、25.85±0.60比23.27±0.92,P＜0.05或P＜0.01);NAC与DEX的作用无差异.结论 NAC可抑制氧自由基损伤及ARDS肺纤维化.

N-acetylcysteine (NAC) inhibited pulmonary fibrosis in acute respiratory distress syndrome (ARDS)

Objective To investigate the effect of NAC treatment on lipopolysaccharide (LPS) treated human embryonic lung fibroblasts (HELF),in regard to oxidant injury and changes in indexes related to pulmonary fibrosis in ARDS.Methods Four groups of cultured HELF were treated with:vehicle,LPS (1 μg/ml),NAC (1 mmol/L)+LPS (1 μg/ml) and dexamethasone (DEX,I μmol/L)+LPS (1 μg/ml) for 24 hours.The content of collagen and the γ-glutamylcysteinylglycine (GSH) in the cells were determined.Results As compared to the control group,the collagen content (μg/mg:78.97士1.79 vs.72.90土1.70)and GSH content (μg/mg:23.27±0.92 vs.26.34土 0.83) in LPS group were significantly (P