| Ang-1对高糖环境中猴脉络膜-视网膜内皮细胞单层通透性的影响 |
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| 引用本文： | 萧鸿,左中夫,冯闯,刘学政. Ang-1对高糖环境中猴脉络膜-视网膜内皮细胞单层通透性的影响[J]. 医学争鸣, 2009, 30(3): 195-198 |
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| 作者姓名： | 萧鸿 左中夫 冯闯 刘学政 |
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| 作者单位： | 萧鸿,XIAO Hong(辽宁医学院,附属第一医院教务科,辽宁,锦州,121001);左中夫,ZUO Zhong-Fu(辽宁医学院,病理生理教研室,辽宁,锦州,121001);冯闯,FENG Chuang(辽宁医学院,研究生学院,辽宁,锦州,121001);刘学政,LIU Xue-zheng(辽宁医学院,人体解剖学教研窜,辽宁,锦州,121001) |
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| 基金项目： | 辽宁省优秀青年科研人才培养资金资助项目，辽宁省科技计划项目 |
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| 摘 要： | 目的：利用猴脉络膜-视网膜内皮细胞建立内皮细胞（EC）单层模型，观察高浓度葡萄糖及血管生成素-1（Ang-1）对该模型通透性的影响．方法：待EC于孔径0．8μm的聚碳酸酯微孔滤膜上形成致密单层后分3组进行培养：对照组（DMEM培养基含25mmol／L葡萄糖）、高糖组（DMEM培养基含30mmol／L葡萄糖）、Ang-高糖组（DMEM培养基含30mmol／L葡萄糖＋0．25mg／L Ang-1）．于1，3，5，7d4个时间点，以含白蛋白1g／L的D—Hanks液灌注滤膜，检测蛋白质渗透压反射系数（8）及滤过系数（K1）；并于5d和7d利用琼脂糖凝胶电泳检测DNAladder．结果：3，5，7d时，高糖组EC单层的通透性高于对照组（P〈0．01）；Ang-高糖组与对照组之间EC单层的通透性无差异（P〉0．05）．高糖组EC的琼脂糖凝胶电泳在5d及7d均出现DNA ladder，对照组及Ang-高糖组未见DNA ladder．结论：高糖能够促进细胞凋亡，增加EC单层通透性；Ang-1能抑制EC的凋亡，对抗高糖所致的EC单层通透性增高．
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| 关 键 词： | 脉络膜 视网膜内皮细胞 血管生成素-1 高糖 通透性 猴 |
Effects of angiopoietin-1 on rhesus macaque choroids-retinal endothelial cells monolayer permeability in high glucose environment |
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| XIAO Hong,ZUO Zhong-Fu,FENG Chuang,LIU Xue-ZhengDivision of Teaching Affairs,First Affiliated Hospital,Depart-ment of Pathophysiology,Postgraduate School. Effects of angiopoietin-1 on rhesus macaque choroids-retinal endothelial cells monolayer permeability in high glucose environment[J]. Negative, 2009, 30(3): 195-198 |
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| Authors: | XIAO Hong ZUO Zhong-Fu FENG Chuang LIU Xue-ZhengDivision of Teaching Affairs First Affiliated Hospital Depart-ment of Pathophysiology Postgraduate School |
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| Affiliation: | XIAO Hong1,ZUO Zhong-Fu2,FENG Chuang3,LIU Xue-Zheng41Division of Teaching Affairs,First Affiliated Hospital,2Depart-ment of Pathophysiology,3Postgraduate School,4Department of Human Anatomy,Liaoning Medical University,Jinzhou 121001,China |
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| Abstract: | AIM: To explore the effects of high concentration glucose and angiopoietin-1(Ang-1) on monolayer permeability of rhesus macaque choroids-retinal endothelial cells(ECs).METHODS: Confluent ECs monolayer was developed on polycarbonate micropore filter.The cells were divided into 3 groups: control group(DMEM containing 25 mmol/L glucose),high glucose group(DMEM containing 30 mmol/L glucose) and Ang-1+ high glucose group(DMEM containing both 30 mmol/L glucose and 0.25 mg/L Ang-1).The ECs were perfused with plain... |
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| Keywords: | choroids retinal endothelial cell angiopoietin-1 high concentration glucose permeability rhesus macaque |
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