人参皂苷Rbl对大鼠局灶性脑缺血白质重塑的影响

目的　探讨人参皂苷Rbl(Ginsenoside Rb1，GSRb1)对大鼠局灶性脑缺血白质重塑的影响。 方法　大鼠随机分为假手术组、溶媒处理组和人参皂苷Rbl处理组，采用线栓法建立大鼠大脑中动脉缺血再灌注（middle cerebral artery occlusion/reperfusion, MCAO/R）损伤模型，用LFB染色观察大鼠胼胝体和内囊的髓鞘变化，用免疫组化染色法检测缺血侧胼胝体GFAP和APP的表达以评估星形胶质细胞和轴突的改变。 结果　缺血2 h再灌72 h后，溶媒处理组胼胝体和内囊有明显的髓鞘紊乱、脱失，胼胝体GFAP和APP表达显著增加。与溶媒处理组相比，GSRb1处理组髓鞘脱失有明显改善 (P<0. 01, P<0. 05)且胼胝体GFAP和APP表达显著减少(P<0. 05)。 结论　GSRb1可能促进大鼠局灶性脑缺血后脑白质重塑。

Effect of ginsenoside Rbl on white matter remodeling in rats with focal cerebral ischemia

Objective To investigate the effect of Ginsenoside Rbl (GSRb1) on white matter remodeling in rats with focal cerebral ischemia. Methods Rats were randomly divided into sham-operated group, vehicle-treated group and GSRb1-treated group, and MCAO/R (middle cerebral artery occlusion/reperfusion) model was induced by thread embolism; then pathological change of myelinsheath was observed by LFB staining in the cerebral corpus callosum and internal capsule, damage to astrocytes and axons was examined by GFAP and APP immunostaining. Results after 72 h followingMCAO/R, obvious demyelination appeared in the corpus callosum and internal capsule and the expression of GFAP and APP was increased significantly in the corpus callosum. Compared with the vehicle-treated group, demyelination was significantly improved in GSRb1-treated group (P<0.01, P<0.05), and the expression GFAP and APP was greatly decreased in GSRb1-treated group (P<0.05). Conclusion Our findings suggest GSRb1 probably promote the white matter remodeling in rats with focal cerebral ischemia.