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Isolation and characterization of polymorphic microsatellite loci for Siphamia tubifer Weber (Perciformes: Apogonidae)
Authors:Tilman J Alpermann  Jörg Plieske  Ahmad O Mal  Ofer Gon  Michael L Berumen
Institution:1. Ichthyology Section, Senckenberg Research Institute and Natural History Museum Frankfurt, Senckenberganlage 25, 60325, Frankfurt am Main, Germany
2. Biodiversity and Climate Research Centre (BiK-F), Senckenberganlage 25, 60325, Frankfurt am Main, Germany
3. TraitGenetics, Am Schwabeplan 1b, 06466, Stadt Seeland OT Gatersleben, Germany
4. Marine Biology Department, Faculty of Marine Sciences, King Abdulaziz University, Jeddah, 21589, Kingdom of Saudi Arabia
5. South African Institute for Aquatic Biodiversity, Private Bag 1015, Grahamstown, 6140, South Africa
6. Red Sea Research Center, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia
Abstract:The cardinalfish Siphamia tubifer has been selected as a model for the study of genetic connectivity in reef-associated fishes among marine-protected-areas in Socotra Island in the northwestern Indian Ocean (part of the Socotra Archipelago, a UNESCO World Heritage Site since 2008). Twenty-six novel microsatellite markers are described for S. tubifer and are now available for studies on its genetic population structure. In a population sample from Socotra Island, the newly developed markers possessed between three and 20 alleles. Expected and observed heterozygosity ranged from 0.56–0.96 to 0.55–0.95, respectively. The markers did not show deviations from Hardy–Weinberg equilibrium and were not in linkage disequilibrium.
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