鳞癌与腺癌患者血清差异蛋白质组分析

目的为鉴定鳞癌与腺癌的血清标志物和病理分型提供依据。方法采用表面增强激光解吸电离飞行时间质谱（SELDI-TOF-MS）技术及其配套的金芯片（Gold Chip）对254份血清（鳞癌120份、腺癌82份,健康人52份）进行蛋白质谱指纹图分析,筛选鳞癌与腺癌的差异蛋白。结果 254份血清共筛选出83个差异表达的蛋白,其中5个差异表达最明显的蛋白质峰的质荷比（m/z）分别为2953、3285、6625、6646、9257。对5个峰强度分别进行F检验,P均〈0.05。结论鳞癌、腺癌患者血清具有明显表达差异的特征蛋白,为癌症的诊断和病理分型研究提供了新思路。

Analysis of serum differential expression proteins in squamous carcinoma and adenocarcinoma

Objective To provide basis for the differention between squamous carcinomas and adenocarcinomas using serum markers and pathomorphological classification.Methods Total 254 serum samples were tested by surface enhanced laser desorption/ionization time of flight mass spectrometry（SELDI-TOF-MS） matched with Gold Chip.There were three groups including 120 squamous carcinomas,82 adenocarcinomas and 52 health controls,the differentia proteins were screened.Results Total 83 differentially expressed proteins were identified,the mass-to-charge ratio（m/z）of 5 protein peaks with the most obvious differential expression was 2 953,3 285,6 625,6 646,9 257,F test showed that all P0.05.Conclusions There exist special protein with differential expression in the patients with squamous carcinoma and adenocarcinoma,this provides a new idea to study serum markers and pathomorphological classification of cancers.