沙棘总黄酮对人脐静脉血管内皮损伤细胞增殖及细胞周期的影响

目的探讨沙棘总黄酮对损伤人脐静脉血管内皮细胞株（CRL-1730）的保护作用。方法采用H2O2诱导CRL-1730细胞损伤模型,并将细胞随机分为四组,正常组不予任何干预;模型组加入250μmol/L H2O2;沙棘组加入沙棘总黄酮终浓度分别为50、100、200μg/m l,继续培养24 h后加入H2O2（剂量同上）;丹参组加入丹参注射液终浓度2.5 mg/m l,继续培养24 h后加入H2O2（剂量同上）。细胞干预4 h后用MTT比色法检测各组细胞活性,并用流式细胞仪测定各组细胞周期。结果与正常组比较,模型组细胞增殖活性下降,S期细胞比率降低,G2/M期细胞比率增加。与模型组比较,沙棘组及丹参组细胞增殖活性升高,G0/G1期细胞比率降低,S期及G2/M期细胞比率均增加。结论沙棘总黄酮对受损血管内皮细胞有保护作用,可提高细胞增殖活性,增加S期细胞比率。

Influence of Total Flavones of Hippophae Rhamnoides L on proliferation and cell circles of HUVEC

Objective To investigate the protective effect of the total flavones of Hippophae Rhamnoides L（TFH） on damaged human umbilical vein endothelial cells（CRL-1730）.Methods Injured model of CRL-1730 were induced by H2O2,and randomly divided into four groups,the normal group were not given any intervention;the model group was added 250 μmol/L H2O2;the TFH groups were added TFH with the final concentrations of 50,100,200 μg/ml respectively,and added H2O2（dose ditto） 24 hs after training;the Danshen group was added Danshen injection with the final concentration of 2.5 mg/ml,and added H2O2（dose ditto） 24 hs after training.Cells were intervened for 4 hs and then determined by MTT colorimetric to detect cell viability,the percentage of cell circles were detected by flow cytometry.Results Compared with the normal group,the cell viability and the percentage of S phase cells decreased,and the percentage of G2/M phase cells increased in the model group;compared with the model group,the cell viability and the percentage of S phase cells,G2/M phase cells in the TFH group and Danshen group increased,and the percentage of G0/G1 phase cells decreased.Conclusions The TFH has precise protective effect on damaged HUVECs,it can raise cell proliferation activity and increase ratio of S phase cells.