| 二次传代法在产前诊断绒毛核型分析中的应用 |
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| 引用本文： | 吴坚柱,陈宝江,谢英俊,林少宾. 二次传代法在产前诊断绒毛核型分析中的应用[J]. 中国优生与遗传杂志, 2011, 0(5): 40-41,56 |
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| 作者姓名： | 吴坚柱 陈宝江 谢英俊 林少宾 |
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| 作者单位： | 中山大学附属第一医院妇产科胎儿医学中心; |
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| 摘 要： | 目的探讨二次传代法在产前诊断绒毛核型分析中的应用价值。方法 210例有产前诊断指征的孕妇在孕11～14w时B超引导下经腹穿刺抽取少量绒毛组织做核型分析。分别采用了一瓶培养法、两瓶培养法和二次传代法三种长期培养法培养绒毛组织,并对其培养时间和培养成功率进行了比较。结果二次传代法平均培养时间为11.1天,比一瓶培养法延长了1.1天,比两瓶培养法缩短了3.5天;二次传代法培养成功率为98.18%,明显高于一瓶培养法(84.0%),略高于两瓶培养法(96.0%)。结论在产前诊断绒毛培养中,二次传代法需要的绒毛标本量少,同时解决了一瓶培养法分裂相少和两瓶培养法培养时间过长的问题,是一种实用的早期产前诊断绒毛核型分析方法。
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| 关 键 词： | 产前诊断 绒毛 二次传代法 |
Application of method of secondary passaging in chorionic villus karyotyping in prenatal diagnosis |
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| WU Jian-zhu,CHEN Bao-jiang,XIE Ying-jun,LIN Shao-bin.. Application of method of secondary passaging in chorionic villus karyotyping in prenatal diagnosis[J]. Chinese Journal of Birth Health & Heredity, 2011, 0(5): 40-41,56 |
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| Authors: | WU Jian-zhu CHEN Bao-jiang XIE Ying-jun LIN Shao-bin. |
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| Affiliation: | WU Jian-zhu,CHEN Bao-jiang,XIE Ying-jun,LIN Shao-bin.(Fetal Medicine Center,Department of Gynaecology and Obstetrics,The First Affiliated Hospital,Sun Yat-sen University,Guangzhou,Guangdong,510080) |
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| Abstract: | Objective:To investigate the value of method of secondary passaging in chorionic villus karyotyping in prenatal diagnosis.Method:210 pregnant women,which had indicatio of prenatal diagnosis,underwent transabdominal chorionic villus sampling via the guidance of B-mode ultrasound at 11~14 weeks for karyotyping.Three long-term culture methods were used to culture chorionic villus,such as one-flask culture method,two-flask culture method and method of secondary passaging.Then compared the culture time and cultu... |
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| Keywords: | Prenatal diagnosis Chorionic villus Method of secondary passaging |
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