| RT-PCR技术在小儿肠道病毒性脑炎诊断中的应用价值探讨 |
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| 引用本文： | 岳欣峰,杨石换.RT-PCR技术在小儿肠道病毒性脑炎诊断中的应用价值探讨[J].中国优生与遗传杂志,2011(5):33-34. |
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| 作者姓名： | 岳欣峰 杨石换 |
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| 作者单位： | 山东荣成市妇幼保健院;荣成市寻山镇医院; |
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| 摘 要： | 目的探讨用RT-PCR技术诊断小儿肠道病毒性脑炎的临床应用价值。方法选取我院2008-06～2009-08期间诊断为病毒性脑炎的住院患儿。于入院的当天抽取脑脊液,经RT-PCR反应,筛选出肠道病毒病例,结果用SPSS13.0进行统计。结果共有45例标本检测出肠道病毒(EV),其中,男20例,检出率76.9%;女25例,检出率83.3%;男女间差异无显著性,(P>0.05)。8月～3岁组16例,检出率80%;3～6岁组18例,检出率81.8%;6～12岁11例,检出率78.6%;各组间差异无显著性,(P>0.05)。EV感染病例与阳性对照可扩增出单一相同的电泳条带;所有阴性对照病例均无扩增。整个检测过程平均用时4.6h。结论①RT-PCR技术可以快速做出病原学诊断,且敏感性高,无非特异性扩增。②该方法操作简单,值得临床上进一步推广。③我院2008-06-2009-08期间诊断为病毒性脑炎的住院患儿以肠道病毒感染为主。④肠道病毒在小儿中为普遍易感,未见有性别及年龄差异性。
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| 关 键 词： | RT-PCR 肠道病毒 病毒性脑炎 小儿 |
Discussing the Application Value of Diagnoseing Children Viral Encephalitis by the RT-PCR Method |
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| YUE Xin-feng,YANG Shi-huan..Discussing the Application Value of Diagnoseing Children Viral Encephalitis by the RT-PCR Method[J].Chinese Journal of Birth Health & Heredity,2011(5):33-34. |
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| Authors: | YUE Xin-feng YANG Shi-huan |
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| Institution: | YUE Xin-feng,YANG Shi-huan.(Department of Pediatrics,The Hospital of Maternal and Child Health in Rongcheng,Rongcheng 264300) |
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| Abstract: | Objective:The aims of the report were to disscuse the clinic value of diagnosing the children enterovirus encephalitis.Methods:We selected the children who were diagnosed worrying viral encephalitis in our hospital from 2008-06~2009-08 and erected the essay group,drawing the CSF at the first day of hospitalization.By the RT-PCR technique,we screen out the EV-infected case.Results:We detected EV RNA from 45 examples.Among the total,there were 20 boys and 25 girls.the corresponding detection rate were 76.9% a... |
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| Keywords: | RT-PCR Enterovirus(EV) Viral encephalitis(VE) Children |
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