| 卵巢早衰microRNA的差异表达及其作用 |
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| 引用本文： | 周莹,朱英哲,张素华,王红梅,王树玉,杨晓葵.卵巢早衰microRNA的差异表达及其作用[J].中国优生与遗传杂志,2011(5):20-22. |
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| 作者姓名： | 周莹 朱英哲 张素华 王红梅 王树玉 杨晓葵 |
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| 作者单位： | 首都医科大学附属北京妇产医院生殖医学科;中国科学院动物所生殖生物学国家重点实验室; |
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| 基金项目： | 国家人事部留学人员科技活动择优资助项目; 北京市优秀人才基金资助(20061D0301000091); 北京市卫生局“十百千”项目 |
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| 摘 要： | 目的探讨卵巢早衰患者（POF）与正常人血浆microRNA（miRNA）表达差异,研究差异miRNA表达与卵巢早衰的关系。方法应用microarray芯片技术检测卵巢早衰患者和正常人血浆中的miRNA表达差异,实时定量PCR（qRT-PCR）验证从microarray芯片中筛选出来的明显差异表达的miRNA。结果 microarray芯片技术结果显示,与正常组比较,卵巢早衰患者的血浆中10种miRNA表达显著上调：mir-202、mir-146a、mir-125b-2、mir-139-3p、mir-654-5p、mir-27a、mir-765、mir-23a、mir-342-3p及mir-126,2种miRNA表达显著下调：let-7c和mir-144。结论卵巢早衰患者和正常人血浆中miRNAs表达谱有差异,这种差异可能与POF发病有关。
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| 关 键 词： | miRNA 卵巢早衰 颗粒细胞 凋亡 |
MicroRNA expression profiles in premature ovarian failure patients and its potential regulate functions |
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| ZHOU Ying,ZHU Ying-zhe,ZHANG Su-hua,WANG Hong-mei,WANG Shu-yu,YANG Xiao-kui..MicroRNA expression profiles in premature ovarian failure patients and its potential regulate functions[J].Chinese Journal of Birth Health & Heredity,2011(5):20-22. |
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| Authors: | ZHOU Ying ZHU Ying-zhe ZHANG Su-hua WANG Hong-mei WANG Shu-yu YANG Xiao-kui |
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| Institution: | ZHOU Ying1,ZHU Ying-zhe1,ZHANG Su-hua1,WANG Hong-mei2,WANG Shu-yu1,YANG Xiao-kui1.(1.Department of Human Reproductive Medicine,Beijing Obstetrics and Gynecology Hospital,Capital Medical University,Beijing,100026,2.State Key Laboratory of Reproductive Biology,Institute of Zoology,Chinese Academy of Sciences,100101) |
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| Abstract: | Objective:To detect the differentially expressed microRNAs(miRNA)from the serum of premature ovarian failure(POF)patients and normal women,and explore the effects of microRNA on POF.Methods:An oligonucleotide microarray chip was used to profile miRNA expressions in POF patients and normal women.The significantly different miRNA,which was selected from microarray chip,validated by quantitative real-time PCR(qRT-PCR).Results:The microarray chip results demonstrated that 10 miRNAs(mir-202,mir-146a,mir-125b-2,m... |
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| Keywords: | miRNA POF Granulosa cells Apoptosis |
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