Expression of angiogenic and fibrogenic factors in proliferative vitreoretinal disorders |
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Authors: | Ahmed M Abu El-Asrar Philippe E Van den Steen Saleh A Al-Amro Luc Missotten Ghislain Opdenakker Karel Geboes |
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Institution: | (1) Department of Ophthalmology, College of Medicine, King Saud University, Riyadh, Saudi Arabia;(2) Laboratory of Molecular Immunology, Rega Institute for Medical Research, University of Leuven, Leuven, Belgium;(3) Department of Ophthalmology, University of Leuven, Leuven, Belgium;(4) Laboratory of Histochemistry and Cytochemistry, University of Leuven, Leuven, Belgium;(5) Department of Ophthalmology, King Abdulaziz University Hospital, Airport Road, P.O. Box 245, Riyadh, 11411, Saudi Arabia |
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Abstract: | Purpose To investigate the expression of connective tissue growth factor (CTGF) in the retina of human subjects with diabetes mellitus,
and CTGF, CD105, and gelatinase B in proliferative diabetic retinopathy (PDR) and proliferative vitreoretinopathy (PVR) epiretinal
membranes.
Methods Twelve donor eyes from six subjects with diabetes mellitus, 10 eyes from five nondiabetic subjects, 14 PDR membranes, and
five PVR membranes were studied by immunohistochemical techniques. In situ zymography was used to examine gelatinolytic activity
in four PDR membranes.
Results In nondiabetic retinas, there was no immunoreactivity for CTGF. Diabetic retinas showed immunoreactivity for CTGF in ganglion
cells and microglia. Vascular endothelial cells in PDR membranes expressed CTGF, CD105, and gelatinase B in 10 (71.4%), 11
(78.6%), and 5 (35.7%) membranes, respectively. Myofibroblasts in PDR membranes expressed CTGF, and gelatinase B in 14 (100%),
and 6 (42.9%) membranes, respectively. There was a significant correlation between the number of blood vessels expressing
the panendothelial marker CD34 and the number of blood vessels expressing CTGF (r = 0.7884; P = 0.0008), and CD105 (r = 0.6901; P = 0.0063), and the number of myofibroblasts expressing CTGF (r = 0.5922; P = 0.0257). There was a significant correlation between the number of myofibroblasts expressing α-smooth muscle actin and
the number of myofibroblaasts expressing CTGF (r = 0.8393; P = 0.0002). In situ zymography showed the presence of gelatinolytic activity in vascular endothelial cells in PDR membranes.
Myofibroblasts in PVR membranes expressed CTGF, and gelatinase B.
Conclusions These results suggest a possible role of CTGF, CD105, and gelatinase B in the pathogenesis of proliferative vitreoretinal
disorders. |
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Keywords: | Diabetic retinopathy CD105 Connective tissue growth factor Gelatinase B Proliferative vitreoretinopathy |
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