大鼠C6脑胶质瘤动物实验模型的建立

目的 为脑胶质瘤的基础理论、临床治疗和药效学研究提供简便、经济、实用的大鼠C6脑胶质瘤动物实验模型。方法 采用自制大鼠脑固定架，固定后，于脑靶点接种C6胶质瘤细胞。观察动物行为状态和生存期，并对脑肿瘤进行形态学，细胞学和分子生物学相关指标的检测。结果 接种C6胶质瘤细胞后，动物进食量减少，体重下降，有的动物出现眶周出血，眼球突出，自身旋转，癫痫发作，偏瘫等症状，大约在2至3周内，动物大多死亡，病理解剖显示颅内肿瘤形成。结论 该方法建立的脑胶质瘤具有与人脑胶质瘤相似的特性，肿瘤形成时间短，存活期较稳定，可供脑胶质瘤基础理论、实验治疗和药效学研究的需要。

Establishment of Rat C6 Glioma Model

Objective To establish an easy, economical, practical rat C6 glioma model.Method After fixation by self-made frame, C6 gliomaous cells were implanted into the specific plot of brain. The behavior and survival time of the rats were observed.Result After inoculation, diminished food intake, weight loss, intraocular hemorrhage, ocular proptosis, per se whirl, epileptic attack and hemiplegia ocurred in model rats. After 2-3weeks, pathological dissection showed oncogenesis in cranium.Conclusion The rat model of brain tumor we made is similar to the human's. The tumor is quick to form, and relatively unchangeable during the survival, so it can be used for studing the tumor's foundational mechanism, experimental cure and pharmacodynamics.