非小细胞肺癌中ETS-1与E-cadherin、N-cadherin、Vimentin表达相关性的研究

目的：探讨ETS-1与E-cadherin、N-cadherin、Vimentin在肺癌中的表达相关性及其临床意义。方法选择接受手术切除并经病理证实的110例非小细胞肺癌（non-small cell lung cancer，NSCLC）患者的肿瘤组织和距肿瘤3 cm以上的癌旁正常肺组织，采用免疫组化SP法检测组织中ETS-1与E-cadherin、N-cadherin、Vimen-tin的表达，并结合临床资料进行相关性分析。结果非小细胞肺癌组织中ETS-1与N-cadherin、Vimentin的阳性表达率分别为65%（72/110）、56%（62/110）、34%（37/110），明显高于正常癌旁肺组织的6%（3/50）、8%（4/50）、2%（1/50），组间差异具有统计学意义（P<0.01）；E-cadherin的阳性表达率为41%（45/110），明显低于正常癌旁肺组织中的84%（42/50）。ETS-1的阳性率与淋巴结转移和TNM分期呈正相关（P<0.05），而与性别、年龄、肿瘤直径、组织学类型及分化程度无关（P>0.05)。E-cadherin的阳性率与肿瘤分化程度、淋巴结转移和TNM分期呈负相关（P<0.05），N-cadherin和Vimentin的阳性率与肿瘤分化程度、淋巴结转移及TNM分期呈正相关（P<0.05），E-cadherin、N-cadherin、Vimentin的表达与患者的性别、年龄、肿瘤直径、组织学类型无相关性（P>0.05）。在非小细胞肺癌中ETS-1与E-cadherin的表达呈负相关，ETS-1与N-cadherin的表达呈正相关。结论 ETS-1与E-cadherin、N-cadherin、Vimentin的表达和非小细胞肺癌的转移密切相关，其检测可作为判断非小细胞肺癌转移和预后的指标之一。

The correlation between expression of ETS-1 and E-cadherin,N-cadherin and Vimentin in non-small cell lung cancer

Objective To observe the expression between ETS-1 and neuronal cadherin (E-cadherin, N-cadherin) and Vimentin in non-small cell lung cancer (NSCLC) tissues and to study the clinical correlation of those molecules. Method 110 cases of NSCLC patients who had undergone surgical resection were confirmed pathologically and were included in this study, of which the tumor tissues and normal lung tissues that were at least 3 cm away from the tumor were selected. The immunohistochemistry SP method was used to detect the expression of ETS-1, E-cadherin, N-cadherin and Vimentin, then the correlation was investigated. Result In NSCLC tissues, the positive expression rate of ETS-1, N-cadherin and Vimentin 65%(72/110), 56%(62/110), and 34%(37/110), respectively] were significant-ly higher than those in normal lung tissues 6%(3/50), 8%(4/50), 2%(1/50), respectively] (all P<0.01); The positive expression rate of E-cadherin was 41%(45/110), which was significantly lower than the 84%(42/50) of normal lung tissues. The expression of ETS-1 was positively correlated with the lymph node metastasis and TNM staging (P<0.05), but was not associated with gender, age, tumor diameter, histological type and differentiation degree (P>0.05). The expression of E-cadherin was negatively correlated with tumor differentiation degree, the lymph node metastasis and TNM staging (P<0.05). The expression of N-cadherin and Vimentin were positively correlated with tumor differ-entiation degree, the lymph node metastasis and TNM staging (P<0.05). The expression of E-cadherin, N-cadherin and Vimentin were not correlated with gender, age, tumor diameter and histological type (P>0.05). While the expres-sion of ETS-1 was negatively correlated with E-cadherin but positively correlated with N-cadherin in NSCLC. Con-clusion The expression of ETS-1, E-cadherin, N-cadherin and Vimentin are closely related with metastasis of NSCLC, thus the detection on the expression of those molecules will be effective in estimating the metastasis and prognosis of NSCLC.