| 动态浊度法定量检测葛根素注射液中细菌内毒素 |
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| 引用本文： | 吴激,孙燕,陈效,刘璟,王云川. 动态浊度法定量检测葛根素注射液中细菌内毒素[J]. 中国药房, 2003, 14(6): 363-364 |
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| 作者姓名： | 吴激 孙燕 陈效 刘璟 王云川 |
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| 作者单位： | 1. 兰州军区乌鲁木齐总医院,乌鲁木齐市,830000
2. 第一军医大学中医系,广州市,510515 |
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| 摘 要： | 目的 :探讨以细菌内毒素检查法取代热原检查法检测葛根素注射液的可行性。方法 :采用定量检测细菌内毒素的动态浊度法 ,用EDS-99细菌内毒素测定仪对葛根素注射液进行抑制增强试验。结果 :标准曲线的回归方程为LogT=2 7957—0 2422LogC ,r=—0 9973 ;葛根素注射液60倍及以上倍数释释对鲎试剂反应无干扰作用。结论 :本方法干扰因素小 ,检测结果准确 ,可作为日常检测使用。
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| 关 键 词： | 葛根素注射液 细菌内毒素 动态浊度法 鲎试剂 |
| 文章编号： | 1001-0408(2003)06-0363-02 |
| 修稿时间： | 2002-06-25 |
Determination of Endotoxin in Injectio Puerarin by Kinetic Turbimetric Assay |
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| WU Ji,SUN Yan,LIU Jing,WANG Yunchuan. Determination of Endotoxin in Injectio Puerarin by Kinetic Turbimetric Assay[J]. China Pharmacy, 2003, 14(6): 363-364 |
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| Authors: | WU Ji SUN Yan LIU Jing WANG Yunchuan |
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| Abstract: | OBJECTIVE:To approach the feasibility of replacing pyrogen test by bacterial endotoxin test(BET)in assaying the injectio puerarin.METHODS:Kinetic turbimetric assay(KTA)was used to determine the bacterial endotoxin,and an inhiˉbition and strengthen test by using the EDS-99bacterial endotoxin systems was adopted for assaying injectio puerarin.RES_ ULTS:The regression equation of standard curve was logT=2.7957—0.2422logC,r=—0.9973;Injectio puerarin did not interfere with limulus test agent in1∶60dilution.CONCLUSION:This method is slight in interference and accurate in deˉtection result,and can be used in routine assay. |
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| Keywords: | injectio puerarin bacterial endotoxin kinetic turbimetric assay limulus test agent |
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