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| 桑叶中降糖活性成分筛选及其药效学研究 | |
| 引用本文： | 李娈,路娟,李永吉,蔡润兰,陈曦. 桑叶中降糖活性成分筛选及其药效学研究[J]. 中医药信息, 2012, 29(5): 10-14 |
| 作者姓名： | 李娈 路娟 李永吉 蔡润兰 陈曦 |
| 作者单位： | 1. 中国医学科学院药用植物研究所,北京100193;黑龙江中医药大学,黑龙江哈尔滨150040 2. 中国医学科学院药用植物研究所,北京,100193 3. 黑龙江中医药大学,黑龙江哈尔滨,150040 |
| 基金项目： | 国家自然科学基金面上项目（81173645）; 国家“重大新药创制”科技重大专项“十二五”课题（2012ZX09301-002-001） |
| 摘 要： | 目的:筛选桑叶中α-葡萄糖苷酶(α-GS苷酶)抑制活性最佳组分,并进行体内降糖活性研究。方法:采用柱层析法对桑叶水提物进行分离,利用体外细胞实验筛选出α-葡萄糖苷酶抑制活性最佳组分,并采用链脲霉素诱导的大鼠高血糖动物模型进行跟踪验证。结果:细胞实验结果表明,桑叶提取物中生物碱的α-葡萄糖苷酶抑制活性最强。体内试验中,与模型组比较,生物碱给药组糖尿病大鼠的血糖有明显降低。结论:桑叶中的生物碱具有显著的降血糖的作用(P0.05)。 |
| 关 键 词： | 桑叶 降血糖 生物碱 α-葡萄糖苷酶 DNJ |
Screening of the Components with Hypoglycemic Activity from Mulberry Leaves and Its Pharmacological Study |
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| LI Luan , LU Juan , LI Yong-ji , CAI Run-lan , CHEN Xi. Screening of the Components with Hypoglycemic Activity from Mulberry Leaves and Its Pharmacological Study[J]. Information on Traditional Chinese Medicine, 2012, 29(5): 10-14 | |
| Authors: | LI Luan LU Juan LI Yong-ji CAI Run-lan CHEN Xi |
| Affiliation: | 1. Chinese Academy of Medical Sciences, Institute of Medical Plant Development, Beifing 100193, China; 2. Heitongjiang University of Chinese Medicine, Harbin 150040, China) |
| Abstract: | Objective：To screen the components of the highest activity inhibiting alpha glucosidase in mulberry leaves, and to investigate the hypoglyeemic activity in vivo. Methods：The aqueous extracts from Mulberry leaves were separated by using column chromatography, and the components of the highest activity inhibiting alpha glucosidase were screened by cell experiments in vitro. The results in vitro were verified in streptozotocin -induced chronic diabetic rats. Results： The results of cell experiments in vitro showed that al- kaloids had the highest inhibitory activity on alpha glucosidase in the mulberry leaf extracts. Compared with model group, blood glucose of diabetic rats in alkaloid group was significantly reduced. Conclusion ： Alkaloids in mulberry leaves have a remarkable hypoglycemic effect（ P 〈 0. 05 ）. |
| Keywords: | Mulberry leaves Hypoglycemic Alkaloid Alpha glucosidase DNJ |
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