芪桂益脉灵对缺血再灌注大鼠IMA表达的实验研究

目的:观察芪桂益脉灵(QGYML)对急性心肌梗死大鼠缺血修饰性白蛋白(IMA)表达的影响。方法:将Wistar大鼠56只,随机分为7组,其中空白对照组(8只)不做任何处理,而假手术组(8只)大鼠开胸后暴露左冠状动脉,不作结扎,余下40只分别分成缺血再灌注组、缺血预适应组、消心痛组、芪桂益脉灵低剂量组及高剂量组,并将大鼠行冠状动脉左前降支结扎造成急性心肌梗死(AMI)模型,模型成功后,分别予QGYML(大、小剂量,即1g/kg、2g/kg)、异山梨酯(消心痛)(2.7mg/kg)和生理盐水,连续灌服7天。采用固相夹心法酶联免疫吸附测定IMA的表达。结果:芪桂益脉灵高剂量组、低剂量组及消心痛组可明显减少缺血再灌注条件下IMA的含量,与缺血再灌注组比较,差异具有统计学意义(P0.05)。结论:芪桂益脉灵可抑制缺血再灌注模型中IMA的表达,具有抗心肌缺血损伤的作用。

Expression of IMAin Rats with Myocardial Ischemia Reperfusion Injury by the Effect of Qigui Yimailing

Objective：To investigate the effects on the Reperfusion Injury by the Qigui Yimailing （QGYML）. expression of IMA in Rats with Myocardial Ischemia Methods ： 56 Wister rats were selected, equi - paroe- cious, and divided into seven groups at random, normal group, sham group, ischemic/reperfusion group （I/ R）, ischemic preconditioning group（IPC）, isosobide dinitarte group （ID）, the low dose of QGYML group and the high dose of QGYML group. Rat models （ n = 40） with AMI were established by deligation of left anterior descending branch, eight rats in sham - operation group （ with thoracotomy and without coronary deligation） and eight rats in control group . The AMI model rats in treatment groups were treated with high - dosage QGYML, low - dosage QGYML and isosorbide dinit rate respectively for seven days, and those in control group were treated with normal saline. The expresstion of IMA in cardiac muscle cells were determined by solidphase phase sandwith enzyme linked immuno sorbent assay（ELISA）. Results： Content of IMA was visibly re- duced on the group of isosobide dinitarte group （ID）, the low dose of QGYML group and the high dose of QGYML group （ P 〈 0. 05 ）. Conclusion ： QGYML has a good and safe action towards ischemic cardiac myocytes （CMCs） ,and can repress the expression of IMA on CMCs. All indicate that it has the anti -cardiac muscle is- chemia damage function.