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| A549细胞条件培养液激活PI3K-Akt1信号通路促人脐静脉内皮细胞存活 | |
| 引用本文： | 涂明利,熊畅,刘先军,罗国仕,杜春玲,徐永健.A549细胞条件培养液激活PI3K-Akt1信号通路促人脐静脉内皮细胞存活[J].肿瘤,2010,30(2). |
| 作者姓名： | 涂明利 熊畅 刘先军 罗国仕 杜春玲 徐永健 |
| 作者单位： | 1. 华中科技大学同济医学院附属同济医院呼吸内科,武汉,430030;郧阳医学院附属太和医院呼吸内科,十堰,442000 2. 郧阳医学院附属太和医院呼吸内科,十堰,442000 3. 华中科技大学同济医学院附属同济医院呼吸内科,武汉,430030 |
| 基金项目： | 湖北省教育厅重点项目 |
| 摘 要： | 目的:研究肺癌A549细胞条件培养液(conditioned medium, CM)对人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVECs)生存活性和凋亡的影响,以及PI3K-Akt信号通路在其中的作用.方法:用制备获得的人肺癌A549细胞CM 培养HUVECs;XTT 法检测HUVECs活性; Hoechst 33258染色,荧光显微镜观察细胞凋亡的形态学改变;FCM法检测细胞的凋亡率; Western印迹法检测Akt总蛋白和磷酸化Akt蛋白表达.分别用特异性PI3K抑制剂 wortmannin(WT)和针对Akt1基因的siRNA (siAkt1)转染HUVECs, RT-PCR法检测Akt亚型mRNA的表达,并观察上述各指标的变化.结果:在CM刺激后24 h, HUVECs活性明显增强(P=0.037),凋亡率下降(P =0.001).CM呈时间依赖性激活HUVECs Akt磷酸化,CM处理后15 min即显示磷酸化Akt水平增高,30 min达高峰,此后呈下降趋势.WT或siAkt1转染均能阻断前述效应.结论:肺癌A549细胞CM经PI3K-Akt途径促进HUVECs存活并抑制其凋亡,其中Akt1起关键作用. |
| 关 键 词： | 癌 非小细胞肺 内皮细胞 信号转导 细胞增殖 细胞凋亡 |
Conditioned medium from lung carcinoma cell line A549 increases the viability of human umbilical vein endothelial cells by activating the PI3K-Akt1 pathway |
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| TU Ming-li,XIONG Chang,LIU Xian-jun,LUO Guo-shi,DU Chun-ling,XU Yong-jian.Conditioned medium from lung carcinoma cell line A549 increases the viability of human umbilical vein endothelial cells by activating the PI3K-Akt1 pathway[J].Tumor,2010,30(2). | |
| Authors: | TU Ming-li XIONG Chang LIU Xian-jun LUO Guo-shi DU Chun-ling XU Yong-jian |
| Institution: | TU Ming-li1,2,XIONG Chang2,LIU Xian-jun2,LUO Guo-shi2,DU Chun-ling2,XU Yong-jian1 (1. Department of Respiratory Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430030,China,2. Department of Respiratory Medicine,Taihe Hospital,Yunyang Medical College,Shiyan 442000,China ) |
| Abstract: | Objective:To study the effects of the conditioned medium (CM) from human lung adenocarcinoma cell line A549 on the viability and apoptosis of human umbilical vein endothelial cells (HUVECs) and the role of PI3K-Akt signaling pathway in the process. Methods:HUVECs were cultured with CM of A549 cells. Cell viability was detected by XTT assay. The morphological changes of HUVECs were analyzed by Hoechst 33258 staining and fluorescence microscopy. The apoptosis was detected by flow cytometry. Expression levels ... |
| Keywords: | Carcinoma non-small cell lung Endothelial cells Signal transduction Cell proliferation Apoptosis |
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