妇科肿瘤细胞的不同制备手段对AFM分析结果的影响

目的 探讨细胞培养技术、细胞印片技术和液基薄层细胞制片技术对原子力显微镜（AFM）分析人卵巢癌细胞和宫颈癌细胞微观形貌的影响。方法 采用细胞培养技术制备人低转移卵巢癌细胞株HO-8910和高转移卵巢癌细胞株HO-8910PM样品，细胞印片技术制备浆液性卵巢癌细胞样品，液基薄层细胞制片技术制备宫颈鳞癌细胞和正常宫颈细胞样品，并采用AFM对3种方法制备的细胞微观形貌进行对比，分析各自特点。结果 3种方法制备的妇科肿瘤细胞样品，AFM观察均显示细胞核增大和胞质分散的形貌特征，以及多个细胞核易聚集和叠加重合，导致细胞尺寸进一步增大的特点。细胞培养技术制备的卵巢癌细胞，细胞呈长方形，整体长度为30～40μm，不能清楚分辨细胞核；细胞印片技术制备的卵巢癌细胞，细胞呈不规则的多边形，胞核为圆形，平均直径为15μm左右；液基薄层细胞制片技术制备的宫颈癌细胞，细胞为裸核、圆形，胞核与周围组织关系孤立，平均直径为30μm。结论 细胞培养技术、细胞印片技术和液基薄层细胞制片技术制备的妇科肿瘤细胞样品均适用于AFM的观察和分析。不同类型的肿瘤细胞受制备方法不同的影响在基底上的形态不同。

Effects of different preparing methods for gynecological cancer cells on analysis results by AFM

Objective To explore effects of different sample preparing methods including cell culture method, cell printing slice method and liquid based cytology detection technology on the morphologies of ovarian cancer and cervical cancer cells by atomic force microscope（ AFM） . Methods Low metastatic ovarian cancer cell line HO-8910 and highly metastatic ovarian cancer cell line HO-8910PM were cultured by cell culture method. The cell printing slice method was used to prepare the serous ovarian cancer sample. The liquid based cytology detection technology was employed to prepare squamous cell carcinoma and normal cervical cells samples. The morphologies of cells prepared by the three methods were obtained by AFM and analyzed. Results For cancer cell samples pre-pared by the three methods, they all showed that the nucleus and cytoplasm increased in the dispersion morphology. Multiple nuclei easily gathered together, and superposition of coincidence caused further increase in size of cells. Main differences of three methods were as follows. For the ovarian cancer cell sample prepared by the cell culturing method, the cells were rectangular shaped, the whole length of the cells was 30-40μm, and the nucleus could not be distinguished clearly. For the ovarian cancer cell sample prepared by the cell printing slice method, the cells were in shape of irregular polygon with a round nucleus and an average diameter was about 15μm. For the squamous cell carcinoma samples prepared by the liquid based cytology detection technology, the samples presented the proper-ties of bare cell nucleus, round nuclei was isolated with the surrounding tissue, and the average diameter was about 30μm. Conclusion Cell culture method, cell printing slice method and liquid based cytology detection technology are completely applicable for gyneco-logical cancer cells observed by AFM tests. Different gynecological cancer cells show different morphologies on the substrate due to the effects of preparation methods.