LRP15基因在胃癌组织中的表达及临床意义

目的 探讨LRP15基因在胃癌中的表达及其与临床病理特征和预后的关系。方法 采用组织芯片技术和免疫组织化学法检测90例胃癌组织及对应癌旁组织中LRP15的表达情况，Kaplan-Meier法进行生存分析， Cox模型评估影响预后的因素。结果 胃癌组织和癌旁组织中LRP15的表达存在显著性差异（0.563±0.046 vs. 0.822±0.054，P＜0.001）。按照胃癌组织和癌旁组织LRP15的比值，将90例患者分为高表达组（比值≥0.5，n=51）和低表达组（比值＜0.5，n=39）。LRP15的表达与肿块大小（P=0.038）、淋巴结转移（P=0.005）有关，与性别、年龄、肿瘤位置、分化程度、大体分型、TNM分期和浸润深度无关（P＞0.05）。高表达者的中位总生存时间为37.3个月，高于低表达者的27.6个月（P=0.040）。Cox模型分析显示，癌组织中LRP15的相对表达量、癌组织与癌旁组织LRP15表达量的比值、淋巴结转移和浸润深度均是影响预后的独立因素。结论 LRP15可能在胃癌生长和淋巴结转移中发挥重要作用，有望成为胃癌预后预测的标志物及靶向治疗的新靶点。

Expression of LRP15 gene in gastric cancer and its clinical significance

Objective To investigate the expression of LRPI5 gene in gastric cancer and its relationship with clinical features and prognosis. Methods The expression of LRPI5 gene was detected using immunohistochemistry in tissue microarrays containing 90 specimens of gastric cancer and paired adjacent normal mucosa tissues. Survival curves were plotted using Kaplan-Meier method, and Cox regression model to evaluate the independent prognostic factors. Results The LRP15 expression was lower in the tumor tissue compared with that in the adjacent normal mucosa tissue （0. 563＋0. 046 vs. O. 822：t=0. 054, P〈O. 001）. According to the ratio of the expression of LRP15 in cancer tissues and that in adjacent tissues were divided into two groups： high-expressian group （ratio〉~O. 5, n = 51 ） and low-expression group （ratio〈O. 5, n = 39）. LRPI5 expression was statistically correlated with the tumor size （P = 0. 038 ） and lymph node metastasis （P = O. 003）, but not with gender, age, tumor location, differentiation, tumor location, gross type, TNM stage and depth of invasion. The Kaplan-Meier method showed that patients with LRP15 low-expression had a significantly shorter medi- an overall survival compared with the patients with high-expression （27.6 vs 37.3 months, P= 0. 040）. Cox multivariate analysis re- vealed that the expression of LRP15 in cancer tissue, ratio of expression between cancer tissue and adjacent normal mucosa tissues, lymph node metastasis and depth of invasion were both independent prognostic factors. Conclusion LRPt5 expression may have an important function in cell growth and is significantly associated with lymph node metastasis. LRP15 can be considered as biomarkers of prognosis and as a new target for gastric cancer therapy.