MTDH和VEGF在上皮性卵巢癌中的表达及临床意义

目的 探讨异黏蛋白（MTDH）及血管内皮生长因子（VEGF）在上皮性卵巢癌中的表达及其与上皮性卵巢癌临床病理特征的关系。方法 采用逆转录 聚合酶链反应（RT-PCR）及免疫组化SP法检测42例上皮性卵巢癌组（恶性组）、20例卵巢良性上皮性肿瘤组织（良性组）及15例正常卵巢上皮组织（正常组）中MTDH和VEGF mRNA及蛋白的表达，分析两者表达与上皮性卵巢癌临床病理特征的关系。结果 MTDH和VEGF mRNA在恶性组中的相对表达量分别为0.672±0.115和0.714±0.129，显著高于良性组（0.324±0.041、0.251±0.039）和正常组（0.317±0.035、0.243±0.031），差异均有统计学意义（P＜0.01）。MTDH及VEGF蛋白在恶性组中的阳性表达率分别为69.0％、80.9％，显著高于良性组（20.0％、35.0％）和正常组（13.3％、20.0％），差异均有统计学意义（P＜0.01）。MTDH和VEGF mRNA及蛋白的表达与上皮性卵巢癌的临床分期、淋巴结转移有关（P＜0.05），而与病理类型及分化程度无关（P＞0.05）。在上皮性卵巢癌中MTDH与VEGF蛋白的表达呈正相关（r=0.420，P＜0.01）。 结论 MTDH和VEGF表达与上皮性卵巢癌的发生、发展有关。

The expression and clinical significance of MTDH and VEGF in epithelial ovarian carcinoma

Objective To explore the expression of metadherin （MTDH） and vascular endothelial growth factor （VEGF） in epithelial ovarian carcinoma （EOC）tissues, and investigate their correlation with clinieopathological characteristics of EOC. Methods The mRNA and protein levels of MTDH and VEGF in 42 samples of EOC tissues, 20 samples of benign epithelial ovarian tomor tissues and 15 samples of normal epithelial ovarian tissues were detected by reverse transeription-polymerase chain reaction （RT-PCR） and im- munohistochemistry. The relationship between MTDH, VEGF and clinicopathologieal characteristics were analyzed. Results The rela- tive expressions of MTDH and VEGF mRNA were 0. 672~0. 115 and 0. 714~0. 129 in EOC tissues, higher than 0. 324＋0. 041 and 0. 251 ~0. 039 in benign epithelial ovarian tumor tissues, and 0. 317~0. 035 and 0. 243e0. 031 in normal epithelial ovarian tissues （P〈 0. 01 ）. The positive rate of MTDH and VEGF protein were 69. 0% and 80. 9% in EOC tissues, significantly higher than 20. 0% and 35.0% in benign epithelial ovarian tumor tissues, and 13.3% and 20. 0% in normal epithelial ovarian tissues（P〈0. 05）. The mRNA levels and positive rates of MTDH and VEGF proteins had correlation with FIGO stages and lymph node metastasis（ P〈O. 05） , but not associated with histological grade and pathological types（ P〉0. 05）. The expression of MTDH had positive correlation with VEGF ex- pression in EOC tissues （r = 0. 420, P〈 0. 01 ）. Conclusion Over-expression of MTDH and VEGF plays an important role the oc- curence and progression of EOC.