广西地不容总生物碱体内抗炎镇痛作用研究

目的 研究广西地不容Stephania kwangsiensis总生物碱（TARS）的体内镇痛抗炎作用。方法 清洁级昆明种小鼠按性别、体质量分层随机分为5组：对照组（等体积蒸馏水），TARS低、中、高剂量（0.15、0.20、0.25 g/kg）组，阿司匹林（阳性对照，0.2 g/kg）组。采用福尔马林致痛法、热水致小鼠缩尾法，观察TARS的镇痛作用；采用小鼠棉球肉芽肿法、蛋清致小鼠足肿胀法，观察其抗炎作用。结果 与对照组比较，高、中、低剂量TARS均可显著降低福尔马林致痛小鼠的疼痛强度，减少小鼠舔后足时间；显著延长热水所致的小鼠缩尾时间；显著减少棉球肉芽组织增生模型中小鼠肉芽肿质量；对蛋清引起的小鼠足肿胀发挥显著抑制作用；且均呈剂量相关性。结论 TARS具有显著的镇痛抗炎作用。

Analgesic and anti-inflammatory effects of total alkaloid in Stephania kwangsiensis in vivo

Objective To study the analgesic and anti-inflammatory effects in vivo of the total alkaloids in Stephania kwangsiensis (TARS). Methods Mice were respectively ig with high, middle, and low doses of TARS. The analgesic effects of TARS were evaluated by the formaldehyde induced pain method and tail-immersion response induced by hot water in mice, and anti-inflammatory effects were observed by cotton ball granuloma, egg white induced paw swelling method in mice. Results TARS could significantly inhibit formaldehyde induced pain in mice, reduce the hind licking times, extend tail withdrawal time induced by hot water, obviously reduced the formation of granulomatous in mice; Inhibited egg white induced paw swelling. Conclusion TARS has significant analgesic and anti-inflammatory effect.