| 血红素加氧酶-1在大鼠原位肝移植急性排斥反应中的作用 |
| |
| 引用本文： | 范伟,张莹,伍晓汀.血红素加氧酶-1在大鼠原位肝移植急性排斥反应中的作用[J].第三军医大学学报,2008,30(17):1654-1657. |
| |
| 作者姓名： | 范伟 张莹 伍晓汀 |
| |
| 作者单位： | 1. 贵州省人民医院肝胆外科,贵阳,550002
2. 四川大学华西医院普通外科,成都,610041 |
| |
| 摘 要： | 目的 探讨血红素加氧酶-1(hemooxygenase-1,HO-1)在大鼠原位肝移植急性排斥反应中的作用.方法 建立大鼠原位肝移植排斥模型后分为3组:对照组(A组),ZnPP组(B组)和CoPP组(C组).3组分别于3、7 d处死,取血及肝脏标本.进行肝功测定(AST、ALT、TBIL、ALB);肝组织HE染色;荧光RT-PCR检测HO-1表达.结果 ①3组肝功能3、7 d时比较有明显的统计学意义(P＜0.05).②HE染色:A组3 d出现I、Ⅱ级为主的排斥反应,7 d时出现Ⅱ级为主的排斥反应;B组3 d出现Ⅱ级为主的排斥反应,7 d时出现Ⅱ、Ⅲ级为主的排斥反应;C组3 d出现排斥反应0～Ⅱ级,以I级为主,7 d时出现排斥反应0～Ⅱ级,以I、Ⅱ级为主.③HO-1在C组表达明显升高,B组不明显,A组介于两组之间.结论 血红素加氧酶-1(HO-1)过表达能减轻急性排斥反应.
|
| 关 键 词： | 血红素加氧酶-1 原位肝移植 大鼠 |
Role of HO-1 in acute rejection after orthotopic liver transplantation in rats |
| |
| FAN Wei,ZHANG Ying,WU Xiao-ring.Role of HO-1 in acute rejection after orthotopic liver transplantation in rats[J].Acta Academiae Medicinae Militaris Tertiae,2008,30(17):1654-1657. |
| |
| Authors: | FAN Wei ZHANG Ying WU Xiao-ring |
| |
| Institution: | FAN Wei1,ZHANG Ying1,WU Xiao-ting2 (1Department of Hepatobiliary Surgery,Guizhou Provincial Hospital,Guiyang 550002,2Department of General Surgery,West China University,Chengdu 610041,China) |
| |
| Abstract: | Objective To investigate the effect of HO-1 on acute rejection after orthotopic liver transplantation (OLT) in rats. Methods Taking dark agouti (DA) rats as donors and Lewis rats as recipients, OLT was performed. Before OLT, the DA rats and Lewis rats were matched in pairs, and divided into control group, ZnPP group and CoPP group, the paired rats of which intraperitoneally received normal saline, 20 mg/kg ZnPP and 5 mg/kg CoPP respectively 24 h before OLT. Ten pairs of rats were in each group. On day 3 or ... |
| |
| Keywords: | hemooxygenase-1 orthotopic liver transplantation rat |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
