Distribution of tenascin-X in different synovial samples and synovial membrane-like interface tissue from aseptic loosening of total hip replacement |
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Authors: | T F Li V Warris J Ma J Lassus T Yoshida S Santavirta I Virtanen Y T Konttinen |
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Affiliation: | (1) Department of Orthopaedics and Traumatology, Helsinki University Central Hospital, Helsinki, Finland, FI;(2) Department of Anatomy, Institute of Biomedicine, University of Helsinki, Helsinki, Finland, FI;(3) Department of Pathology, Mie University School of Medicine, Mie, Japan, JP;(4) Division of Rheumatic Diseases, Department of Medicine, Helsinki University Central Hospital, Helsinki, Finland, FI;(5) Department of Oral Medicine, Helsinki University Central Hospital, Helsinki, Finland, FI |
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Abstract: | The distribution of tenascin-X (Tn-X) was investigated in synovial samples from rheumatoid arthritis (RA), osteoarthritis
(OA) and knee injuries, and in synovial membrane-like interface tissue (SMLIT) from aseptic loosening of total hip replacement
(THR). An affinity purified rabbit antiserum against Tn-X was applied in avidin-biotin-peroxidase complex method. Double immunofluorescence
labeling was used to assess the spatial relationship of Tn-X and Tn-C. All samples showed Tn-X immunoreactivity. Strong staining
appeared in the lining and lining-like layers of RA and SMLIT samples, respectively. An intensive immunoreactivity was also
found in pannus tissue in RA, and around multinucleate giant cells and polyethylene wear debris in SMLIT. Staining intensity/extent
varied significantly in different samples in the following rank order: SMLIT, RA, OA, knee synovium membrane. Double labeling
revealed two patterns of Tn-X/Tn-C distribution, reciprocal and co-localization. Our results suggest that Tn-X is an essential
component of normal synovial membrane, and that inflammatory mediators may increase local Tn-X production. Tn-X distribution
is not always reciprocal to that of Tn-C.
Received: 16 July 1999 / Accepted: 20 January 2000 |
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Keywords: | Tenascin Total hip replacement Interface tissue Rheumatoid arthritis Osteoarthritis |
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