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| 实时定量PCR测定大肠癌细胞系中ERCC1 mRNA水平及草酸铂药物抵抗的关联性分析 | |
| 引用本文： | 逯晓波,王雪莲,李晓霞,刘嵘,安春丽. 实时定量PCR测定大肠癌细胞系中ERCC1 mRNA水平及草酸铂药物抵抗的关联性分析[J]. 沈阳药科大学学报, 2008, 25(5): 404-408 |
| 作者姓名： | 逯晓波 王雪莲 李晓霞 刘嵘 安春丽 |
| 作者单位： | 1. 中国医科大学,公共卫生学院,辽宁,沈阳,110001 2. 中国医科大学,基础医学院,辽宁,沈阳,110001 3. 中国医科大学,第四临床医院大肠外科,辽宁,沈阳,110035 |
| 摘 要： | 目的分析草酸铂处理前后ERCC1 mRNA水平的变化,探讨肠癌细胞中ERCC1表达与草酸铂药物抵抗性的关联。方法以标准化肠癌细胞为研究模型,SRB细胞抑制率实验评价不同肠癌细胞对草酸铂的敏感性,实时定量PCR测定草酸铂处理前后的变化。结果不同肠癌细胞对草酸铂的敏感性不同。草酸铂处理前后ERCC1 mRNA水平变化不明显。无论草酸铂处理前后,IC50与ERCC1 mRNA水平呈正相关。结论肠癌细胞对草酸铂的敏感性与肠癌细胞中固有的ERCC1表达水平密切相关,可能作为预测草酸铂使用有效性与安全性的分子标志物。 |
| 关 键 词： | 切除修复交叉互补集团1基因(ERCC1) 大肠癌细胞系 草酸铂 药物抵抗性 |
| 文章编号： | 1006-2858(2008)05-0404-05 |
| 修稿时间： | 2007-06-21 |
Study on relationship between oxaliplatin antitumor resistance and ERCC1mRNA level in colorectal carcinoma cell lines |
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| LU Xiao-bo,WANG Xue-lian,LI Xiao-xia,LIU Rong,AN Chun-li. Study on relationship between oxaliplatin antitumor resistance and ERCC1mRNA level in colorectal carcinoma cell lines[J]. Journal of Shenyang Pharmaceutical University, 2008, 25(5): 404-408 | |
| Authors: | LU Xiao-bo WANG Xue-lian LI Xiao-xia LIU Rong AN Chun-li |
| Abstract: | Objective To analyze ERCC1 mRNA level before and after oxaliplatin treatment,and find the relationship between oxaliplatin anti-tumor resistance and ERCC1 mRNA level.Methods Standard colorectal carcinoma cell lines to oxaliplatin sensitivity were measured by SRB assay,and ERCC1 mRNA levels in all cell lines were measured by real-time PCR.Results Sensitivities to oxaliplatin in different cell lines were different.There was a positive relationship between IC50 and ERCC1mRNA in all cell lines whether before or after oxaliplatin treatment.Conclusions Sensitivities to oxaliplatin are closely related to inherent ERCC1 mRNA levels in colorectal cell line;ERCC1 is an important molecular marker to predict validity and security of oxaliplatin treatment. |
| Keywords: | ERCC1 colorectal carcinoma cell line oxaliplatin antitumor resistance |
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