Characterization of protein in old myocardial infarction by FTIR micro-spectroscopy |
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Authors: | Na ZHENG Tiantong YANG Man LIANG Haidong ZHANG Liping LI Ananda Sunnassee Liang LIU |
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Institution: | 1. Faculty of Forensic Medicine,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China 2. Key Laboratory of Evidence Science(China University of Political Science and Law),Ministry of Education,Beijing 100040,China |
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Abstract: | The aim of the present study was to assess whether Fourier transform infrared spectrometry (FTIR) micro-spectroscopy could
produce distinct spectral information on protein of old myocardial infarction (OMI) and to set them as molecular markers to
diagnose atypical OMI. Paraffin-embedded heart samples were derived from victims dying of OMI. In combination with histological
stain, FTIR and infrared micro-spectroscopy, the characteristics of OMI were analyzed morphologically and molecularly. The
most relevant bands identified were the amide A, B, I and, II showing crucial spectral differences between apparent normal
region and OMI region, including the peak position blue shift and the increased intensity of OMI, moreover relative increase
in α-helix and decrease in β-sheet of protein secondary structures in OMI. Comparing to single spectral band, the I1650/I1550 ratio was increased and rationally used as a molecular marker for diagnosing OMI. These novel preliminary findings supported
further exploration of FTIR molecular profiling in clinical or forensic study, and were in accordance with histopathology. |
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Keywords: | Fourier transform infrared spectrometry spectral pathology old myocardial infarction protein structure molecular characteristics |
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