Solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of IgG rheumatoid factor-secreting cells |
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| Authors: | A Tarkowski C Czerkinsky L A Nilsson H Nygren O Ouchterlony |
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| Affiliation: | 1. Department of Medical Microbiology, University of Göteborg S-413 46 Göteborg, Sweden;2. Department of Histology, University of Göteborg, S-400 33 Göteborg, Sweden;3. Department of Rheumatology, Sahlgrenska Hospital, S-413 45 Göteborg, Sweden |
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| Abstract: | Although IgG rheumatoid factor may play an essential role in the pathogenesis of rheumatoid arthritis, there is no precise method for its specific detection at the cellular level. A modification of the recently developed enzyme-linked immunospot assay has been devised for enumeration of cells secreting IgG rheumatoid factor (IgG RF) and simultaneous quantitation of the IgG RF secreted. Specific, sensitive and simple, this new assay should provide a valuable tool for study of isotype-specific RF secretion by single cells. |
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| Keywords: | IgG rheumatoid factor-secreting cells immunospot assay (ELISPOT) |
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