ERK表达及活化在自发性高血压大鼠心肌肥厚中作用的研究

目的　以SHR大鼠作为自发性高血压动物模型 ,研究ERK表达及活化在高血压并发左心室肥厚 (LVH)中的作用。方法　SHR大鼠按年龄分为 8周、16周和 2 4周三组 ,以Wistar大鼠作为对照。ERK表达及活性定量测定采用WesternBlot方法。结果　SHR左室质量指数与磷酸化ERK水平正相关。 2 4周龄SHR大鼠基础ERK表达水平较 8周龄和 16周龄减少 ,亦明显低于同龄Wistar大鼠 (P =0 0 0 3) ;SHR大鼠ERK活化程度高于同龄Wistar大鼠 ,随年龄增加 ,SHR磷酸化ERK表达量增加。结论　ERK的活化参与高血压心肌肥厚的发病。

Extracellular Signal Regulated Kinase(ERK) in Essential Hypertension and Myocardial Hypertrophy

Objective To assess the roles of ERK expression and activity in hypertensive myocardial hypertrophy in spontaneously hypertensive rats(SHR). Methods SHRs were divided into 3 groups according to age,Wistar rats acted as controls. Quantitative measurement of ERK expression and activity in myocardium was performed by Western blot. Results Phospho-ERK was positively related to LVMI. The basal ERK expression in myocardium was decreased in 24 wk old SHR compared with 8 wk and 16 wk rats. It's concentration was significantly lower than the age matched WKY as well. Basal-ERK expression decreased with age,while phospho-ERK increased. Conclusion The activation of phospho-ERK play some role in the development of hypertensive myocardial hypertrophy.