苯中毒与热应激蛋白抗体关系的研究

为了探讨苯中毒与热应激蛋白抗体水平的关系．采用Cometassay和Westerblot等方法对不同浓度苯接触的工人进行了外周血白细胞计数、血红蛋白、血清超氧化物酶活性、淋巴细胞DNA损伤、热应激蛋白（HSP）27、60、71和90抗体进行了测定。结果发现：苯中毒工人血浆中HSP71抗体高发生率与其白细胞减少、血红蛋白降低、血清SOD活性和淋巴细胞DNA损伤增加是相联系的。这些结果表明HSP71抗体可能参与了苯中毒的发生和作为苯中毒监测指标的可能性。

Study on the relationship between benzene poisoning and antibodies against heat stress proteins

In order to explore the relationship between benzene poisoning and antibodies against heatstress proteins (HSPs) .it was determined that the number of white blood cells,the content of hemoglobin,theactivities of superoxide dismutase (SOD). Iymphocyte DNA damage and presence of antibodies to differentHSPs (HSP27. HSP60,HSP71and HSP90)in blood samples from the workers exposed to a different concentration of benzene with Comet assay and Western blot. The results showed that the high incidence of antibodiesagainst HSP71 in benzene -- poisoned workers was associated with a decrease in white blood cells andhemoglobin,and with an increase in activities of serum SOD and lymphoctyte DNA damage. These resultssuggested that antibodies against HSP71 might mediate the formation of benzenepoisoning and be a possiblebiomarker for evaluating benzene--poisoning.