| 低分子季铵化壳聚糖对乳腺癌细胞作用的研究 |
| |
| 引用本文： | 刘其凤,任慧霞,薛伟华.低分子季铵化壳聚糖对乳腺癌细胞作用的研究[J].中国生化药物杂志,2006,27(1):35-37. |
| |
| 作者姓名： | 刘其凤 任慧霞 薛伟华 |
| |
| 作者单位： | 山东大学,药学院,生化与生物技术药物研究所,山东,济南,250012 |
| |
| 摘 要： | 目的研究低分子季铵化壳聚糖对MCF-7乳腺癌细胞的作用,探讨其抑制肿瘤的作用机理。方法应用四甲基偶氮唑蓝(MTT)法、流式细胞仪检测低分子季铵化壳聚糖对MCF-7乳腺癌细胞生长的影响,采用端粒酶试剂盒考察其对端粒酶的影响。结果低分子季铵化壳聚糖可抑制MCF-7乳腺癌细胞的生长,作用于细胞周期G1期,抑制端粒酶活性。结论低分子季铵化壳聚糖能够诱导MCF-7乳腺癌细胞的凋亡,具有一定的抑瘤作用。
|
| 关 键 词： | 低分子季铵化壳聚糖 MCF-7乳腺癌细胞 细胞凋亡 |
| 文章编号： | 1005-1678（2006）01-0035-03 |
| 收稿时间： | 2005-04-05 |
| 修稿时间： | 2005-07-31 |
Effect on MCF-7 breast cancer of low molecular N-trimethyl chitosan chloride |
| |
| LIU Qi-feng,REN Hui-xia,XUE Wei-hua.Effect on MCF-7 breast cancer of low molecular N-trimethyl chitosan chloride[J].Chinese Journal of Biochemical Pharmaceutics,2006,27(1):35-37. |
| |
| Authors: | LIU Qi-feng REN Hui-xia XUE Wei-hua |
| |
| Institution: | Institute of Biochemical and Biotechnological Drug, School of Pharmacy, Shandong University, Jinan 250012, China |
| |
| Abstract: | Purpose To study the effect on MCF-7 breast cancer and to investigate the anti-cancer mechanism of low molecular N-trimethyl chitosan chloride. Methods The effect on MCF-7 breast cancer cell growth of low molecular N-trimethyl chitosan chloride was detected by MTT method and flow cytometry. And the influence on telomerase of low molecular N-trimethyl chitosan chloride was investigated by using telomerase kit. Restilts Low molecular N-trimethyl chitosan chloride had the activity of suppressing the MCF-7 breast cancer eell growth in cell cycle G1 and inhibiting telomerase activation. Conclusion low molecular N-trimethyl chitosan chloride could induce the apoptosis of MCF-7 breast cancer and had the anti-cancer activity. |
| |
| Keywords: | low molecular N-trimethyl chitosan chloride MCF-7 breast cancer apoptosis |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
