| 重组sICAM-1对PMA刺激的白细胞与=肺微血管内皮细胞粘附的影响 |
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| 引用本文： | 孔炜,于江洲,王迪浔. 重组sICAM-1对PMA刺激的白细胞与=肺微血管内皮细胞粘附的影响[J]. 中国病理生理杂志, 2001, 17(5): 431-434 |
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| 作者姓名： | 孔炜 于江洲 王迪浔 |
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| 作者单位： | 同济医科大学病理生理教研室,湖北武汉430030 |
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| 基金项目： | 卫生部科研基金资助(No.96-1-137) |
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| 摘 要： | 目的:探讨重组sICAM-1对大鼠白细胞与肺微血管内皮细胞粘附的影响。方法:采用原代培养的大鼠肺微血管内皮细胞及99Tm-HMPAO标记的白细胞,观察不同浓度的sICAM-1、CA7(sICAM-1的单抗)以及sICAM-1·CA7(sICAM-1的二聚体)对PMA刺激的大鼠白细胞与肺微血管内皮细胞粘附的影响。结果:sICAM-1与CA7即使在100mg/L也不能抑制白细胞与肺微血管内皮细胞的粘附,而其二聚体在20mg/L和40mg/L即可抑制42%和50%的细胞粘附(P＜0.05)。结论:sICAM-1对细胞粘附的抑制作用很弱,可能与其单体形式有关。
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| 关 键 词： | 细胞粘着分子 白细胞 粘连 |
| 文章编号： | 1000-4718(2001)05-0431-04 |
| 收稿时间： | 1999-12-22 |
| 修稿时间： | 1999-12-22 |
Effect of recombinedsICAM-1 on the adhesion of leukocyte to pulmonary microvascular endothelial cellstimulated by PMA |
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| KONG Wei,YU Jiang-zhou,WANG Di-xun. Effect of recombinedsICAM-1 on the adhesion of leukocyte to pulmonary microvascular endothelial cellstimulated by PMA[J]. Chinese Journal of Pathophysiology, 2001, 17(5): 431-434 |
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| Authors: | KONG Wei YU Jiang-zhou WANG Di-xun |
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| Affiliation: | Department of Pathophysiology, Tongji Medical University,Wuhan 430030, China |
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| Abstract: | AIM:To explore the effect of recombined sICAM-1 on the adhesion of leukocyte to pulmonary microvascular endothelial cell. METHODS:Primary cultured rat pulmonary microvascular endothelial cells were used in this experiment. Leukocyte was labeled with 99 Tm-HMPAO.The effects of different dose of sICAM-1, CA7 and dimeric form of sICAM-1 (sICAM-1*CA7)were tested separately in cell adhesion .RESULTS:sICAM-1 and CA7 could not inhibit cell adhesion even at the concentration of 100 mg/L,while sICAM-1*CA7 at concentrations of 20 mg/L and 40 mg/L could inhibit 42% and 50% of cell adhesion respectively (P＜0.05). CONCLUSION:sICAM-1 has poor inhibitory effect on cell adhesion, probably due to monomeric form. |
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| Keywords: | Cell adhesion molecules Leukocytes Adhesions |
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