益骨胶囊对去卵巢骨质疏松大鼠骨组织cbf α-1mNRA表达的影响

目的： 分析成骨细胞分化因子cbf α-1基因在去卵巢骨质疏松（OP）大鼠的表达水平，观察益骨胶囊的调节作用。方法: 将36只10月龄SD雌性大鼠随机分为假手术组、模型组、药物组,相应干预后,留取股骨,采用SYBR GreenⅠ荧光定量PCR技术测定cbf α-1 mRNA表达,以GAPDH基因为内参照,根据相对定量公式(2-ΔΔCt)分别计算模型组、药物组与假手术组表达差异倍数。结果: 根据相对定量公式分析显示:与假手术组相比，模型组大鼠骨组织中cbf α-1 mRNA表达下降（P<0.01）。而药物组cbf α-1 mRNA表达相当于假手术组的0.19－0.92倍(P>0.05)，明显高于模型组（P<0.05）。结论: 结果表明去卵巢OP模型大鼠骨组织cbf α-1基因表达降低，而益骨胶囊可上调cbf α-1的基因表达水平，这可能与其诱导骨髓间质干细胞分化为成骨细胞发挥其防治OP的作用有关。

Effects of Yigu capsule on the level of cbf α-1 mRNA in bone of ovariectomized osteoporosis rats

AIM: To analyze the regulatory effect of Yigu capsule on core binding factor alpha 1 (cbf α-1) gene expression in bone of ovariectomized osteoporosis (OP) rats. METHODS: Thirty-six 10-month old Sprague-Dawley female rats were randomized into three groups: sham-operated group, model group and drug group. After intervention by the corresponding methods, the femurs were collected, SYBR green Ⅰ fluorescence quantitative PCR technique was applied with the internal control of GAPDH according to the relative quantitative formula (2-ΔΔCt), the differentially expressed multiples between the model group or drug group and sham-operated group were calculated. RESULTS: Quantitative formula analysis showed that the level of cbf α-1 gene expression in bone of model groups was decreased than that in sham-operated rats ［compared with sham-operated group, P<0.01, it was (9.9×105)-(1.6×104) times］. While in drug groups the level of cbf α-1 gene expression was 0.19 to 0.92 times than that in the sham-operated group, no significant difference was observed (P>0.05). CONCLUSION: The results of this study show that cbf α-1 gene expression in bone tissue of ovariectomized OP rat is decreased, and Yigu capsule increases the level of cbf α-1 gene expression in bone of OP, indicating that Yigu capsule induces bone marrow mesenchymal stem cells into osteoblasts.