1，3-二苯-1，3-丙二酮对可卡因致小鼠神经递质含量变化的影响

目的：探讨1,3-二苯-1,3-丙二酮（1,3-diphenyl-1,3-propanedione，DPPD）对可卡因所致小鼠神经递质含量变化的影响。方法： 36只雄性健康的ICR小鼠随机分为对照组、可卡因组、3个DPPD预给药组（200、400、800 mg/kg）和DPPD单独给药组（800 mg/kg）。对照组灌胃给予1%（体积分数）吐温3 d；可卡因组灌胃给予1%吐温2 d，第3天皮下注射可卡因；3个DPPD预给药组小鼠分别灌胃给药（DPPD 200、400、800 mg/kg）3 d，第3天给药30 min后皮下注射可卡因；DPPD单独给药组用DPPD 800 mg/kg灌胃给药3 d。给予可卡因20 min后处死小鼠，采用高效液相色谱法 荧光检测器检测小鼠脑组织中多巴胺（dopamine，DA）和5-羟色胺（5-hydroxytryptamin，5-HT）的含量，采用高效液相色谱法 紫外检测器检测小鼠脑组织中谷氨酸（glutamic acid，Glu）和γ 氨基丁酸（γ-aminobutyric acid，GABA）的含量，比较各组间神经递质的水平。结果： 与对照组相比，可卡因组小鼠脑中DA和GABA含量明显升高（P<0.01和P<0.05），Glu含量显著降低（P<0.05）。与可卡因组相比，DPPD预给药组（200、400、800 mg/kg）小鼠脑中DA含量明显下降（P<0.01），DPPD预给药200 mg/kg组小鼠脑中GABA含量明显下降（P<0.05）。DPPD预给药200 mg/kg组小鼠脑中DA、5-HT、Glu和GABA含量与对照组的差异均无统计学意义。结论： 低剂量DPPD可逆转可卡因致小鼠神经递质含量的改变。

Effects of 1,3-diphenyl-1,3-propanedione on neurotransmitter contents of brain in mice administered with cocaine

Objective:To investigate the possible reversal effects of 1 ,3-diphenyl-1 ,3-propanedione (DPPD)for cocaine-induced content changes of neurotransmitters of brain in mice.Methods:In this study,36 healthy ICR male mice were randomly divided into control group,cocaine group,three DPPD pretreatment groups (200,400,and 800 mg/kg)and DPPD alone group (800 mg/kg).The mice in control group were administered intragastrically with 1 % Tween 80 for 3 d,and the mice in cocaine group were administered intragastrically with 1 % Tween 80 for 2 d before cocaine was injected subcutaneously on the 3rd day.The mice in the three DPPD pretreatment groups were administered intragastrically (DPPD 200,400,and 800 mg/kg)for 3 d before cocaine was injected subcutaneously 30 min after the administration on the 3rd day.The mice in DPPD alone group were administered intragastrically with DPPD at dose of 800 mg/kg for 3 d.The mice were sacrificed 20 minutes after cocaine injection.The contents of dopamine (DA)and 5-hydroxytryptamin (5-HT)in the mice brain were determined by high performance liquid chromatography (HPLC)-fluorescence detector,the contents of glutamic acid (Glu) and γ-aminobutyric acid (GABA)in the mice brain were determined by HPLC-ultraviolet detector,and the neurotransmitter levels were compared between the groups.Results:The results showed that as com-pared with the control group,DA and GABA contents in cocaine group increased significantly (P <0.01 and P <0.05),while Glu content decreased (P <0.05).As compared with cocaine group,the DA levels in the three DPPD pretreatment groups (200,400,and 800 mg/kg)all decreased significantly (P <0.01 ).In DPPD 200 mg/kg pre-administration group,GABA content decreased (P <0.05),and the contents of the four kinds of neurotransmitters had no statistical differences with those of the control group.Conclusion:DPPD may have potential reversal effects of the content changes of neurotransmitters in mice brain induced by cocaine at a lower dose.