Quantification of the morphological features of a full microvascular network |
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Authors: | Mr S B Bidiwala M S Mansour C K Stengel S A Klein S M Carroll S C Koenig A H Desoky G R Tobin C Maldonado J H Barker |
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Institution: | (1) Division of Plastic & Reconstructive Surgery, University of Louisville, 40292 Louisville, KY, USA;(2) Department Engineering Math & Computer Science, University of Louisville, 40292 Louisville, KY, USA;(3) Department of Plastic & Reconstructive Surgery, Cork Regional Hospital, Cork, Ireland;(4) Division of Cardiothoracic Surgery, University of Louisville, 40292 Louisville, KY, USA |
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Abstract: | Investigations into the changes that occur in microvasculature following the surgical procedure called delay have brought
about the need for a computer system capable of quantifying the morphological features of a full microvascular network in
terms of average vessel length, diameter, and tortuosity. Both the formulaic conventions that have been developed to measure
these quantities as well as their implementation in the form of a HP-9000/UNIX based computer software system that we developed
specifically for this purpose are discussed. Reliability studies performed using the final system to measure the microcirculatory
network of a mouse latissmus dorsi muscle (LDM) showed 95% confidence intervals within 5% of means and coefficients of variability
within 7% of means for all quantities measured in large (150–300 μm), medium (50–150 μm), and small (<50 μm) diameter vessels.
These variations were significantly smaller than the changes that were observed in a preliminary study comparing these microvascular
network parameters before and after delay in the hairless mouse LDM, showing the proposed quantification methods to be well
suited to the study of the microvascular changes following delay. It is hoped that the formulaic conventions, implementation
process and reliability data will provide a useful comparison for other researchers interested in measuring similar features
of microcirculatory networks. |
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Keywords: | Microcirculation Diameter Length Tortuosity |
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