ASSAY OF S100 LANGERHANS' CELLS IN CERVICAL CARCINOMA

Langerhans cells (LCs) in the normal cervix (control, 19 cases), cervical carcinoma in situ( CIS, 19 cases) and invasive cervical carcinoma (30 cases), interdigitatlng cells (IDCs) and follicular dendritic cells (FDCs) in obturator lymph node draining Invasive cervical carcinoma were quantitatively assessed by ABC immunohistochemical staining using anti-S100 protein antibody. The results indicated that S100 LCs in sltu, S100 IDCs and S100 FDCs In obturator lymph node showed dendritic features with a specific distribution. Number of LCs in situ in Invasive carcinoma increased significantly when compared with CIS and control groups. There was no change in LCs number between grade Ⅱ and Ⅲ of squemous cell carcinoma. Number of IDCs was significantly less in stage Ⅱ than in stage Ⅰ . FDCs number In different Invasive depthes and clinical stages showed no obvious change. The results suggest that progression of cervical carcinoma is closely related to decrease of LCs hi situ and IDCs in regional lymph n

Assay of S100+ langerhans’ cells in cervical carcinoma

Langerhans cells (LC_s) in the normal cervix (control, 19 cases), cervical carcinoma in situ( CIS, 19 cases) and invasive cervical carcinoma (30 cases), interdigitating cells (IDC_s) and follicular dendritic cells (FDC_s) in obturator lymph node draining invasive cervical carcinoma were quantitatively assessed by ABC immunohistochemical staining using anti-S100 protein antibody. The results indicated that S100⁺ LCs in situ, S100⁺ IDCs and S100⁺ FDCs in obturator lymph node showed dendritic features with a specific distribution. Number of LCs in situ in invasive carcinoma increased significantly when compared with CIS and control groups. There was no change in LCs number between grade I and III of squemous cell carcinoma. Number of IDCs was significantly less in stage II than in stage I . FDCs number in different invasive depthes and clinical stages showed no obvious change. The results suggest that progression of cervical carcinoma is closely related to decrease of LCs in situ and IDCs in regional lymph node. Predominant immune response of the host to cervical carcinoma should be cellular immunity.