血小板的软X射线谱学显微成像研究

探索性地应用软 X 射线谱学显微技术研究血小板的结构及钙元素成像的变化，采用谱学显微术对制取的 SD 大鼠血小板形态扫描透射成像并对血小板作钙元素双能对比衬度成像。血小板成像可看到血小板大小不等，有的伸出伪足；钙元素分布成像血小板在静息状态时胞内钙元素主要分布于质膜侧凹陷的钙池，诱导聚集后则扩散分布于胞质。软X 射线谱学显微成像能反应血小板钙元素分布，为钙的探测提供一种新方法。

Microspectroscopic soft X-ray imaging analysis of platelet

To test the transformation of intracellular calcium in platelet initially applying microspectroscopy soft X-ray for imaging based on the truth the platelets were at the status of activation in a variety of illness. Normal adult SD rats were enrolled in the trial and finally the rat platelets were extracted for platelet scanning transmission and dual-energy calcium contrast imaging. From the morphology and distribution of calcium of platelet,a various type of platelet morphology pictures were observed,of which the cell deformed and the parapodium stretched out;while calcium mainly distributed in the calcium pool near plasmalema under quiescent condition but scattered across the cytoplasm in activated condition induced by adenosine diphosphate. In conclusion,the microspectroscopy soft X-ray imaging was capable of reflecting the distribution of calcium in the platelet and provides a practicable new method for the measure of intracellular calcium.