| 半套式PCR扩增人抗体基因以增加抗体库的多样性 |
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| 引用本文： | 王学,王海涛.半套式PCR扩增人抗体基因以增加抗体库的多样性[J].军事医学科学院院刊,1996,20(1):45-48. |
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| 作者姓名： | 王学 王海涛 |
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| 作者单位： | 军事医学科学院微生物学流行病学研究所 |
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| 摘 要： | 用重链５’－末端８条引物和３’末端１条引物以及轻链５’末端９条引物和３’末端２条引物ｃＤＮＡ为模板对免疫球蛋白基因进行了ＰＣＲ扩增。结果表明，重链的扩增率为５０％，轻链的扩增率为４４％。而先用信号肽序列为经物和以上３’末端引物以ｃＤＮＡ为模板先进行第一次ＰＣＲ，然后以该产物为模板再用以上方法进行第二次ＰＣＲ时，不仅所有的引物都佑欣?
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| 关 键 词： | 抗体库 抗体基因 半套式 聚合酶链反应 引物 |
Amplification of human antibody genes with half nested PCR to increase the diversity of antibody library |
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| Wang Xue,Wang Haitao,Chen Wanrong,Bao Zuoyi,Xu Jing Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing.Amplification of human antibody genes with half nested PCR to increase the diversity of antibody library[J].Bulletin of the Academy of Military Medical Sciences,1996,20(1):45-48. |
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| Authors: | Wang Xue Wang Haitao Chen Wanrong Bao Zuoyi Xu Jing Institute of Microbiology and Epidemiology Academy of Military Medical Sciences Beijing |
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| Institution: | Wang Xue,Wang Haitao,Chen Wanrong,Bao Zuoyi,Xu Jing Institute of Microbiology and Epidemiology,Academy of Military Medical Sciences,Beijing 100071 |
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| Abstract: | |
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| Keywords: | half nested PCR primer design antibody library polymorphism polymerase chain reaction antibodies monoclonal immunoglobulins |
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